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OriGene Antibodies in recent publications
A common polymorphism in the LDL receptor gene has multiple effects on LDL receptor function Hum. Mol. Genet., Apr 2013; 22: 1424 - 1431. [anti-DDK]
Excitotoxicity Upregulates SARM1 Protein Expression and Promotes Wallerian-Like Degeneration of Retinal Ganglion Cells and Their Axons Invest. Ophthalmol. Vis. Sci., Apr 2013; 54: 2771 - 2780. [SARM1]
Modulation of TET2 expression and 5-methylcytosine oxidation by the CXXC domain protein IDAX Nature 497, 122-126 doi:10.1038/nature12052 [anti-DDK]
Natriuretic Peptide Receptor-3 Gene (NPR3): Nonsynonymous Polymorphism Results in Significant Reduction in Protein Expression Because of Accelerated Degradation Circ Cardiovasc Genet, Apr 2013; 6: 201 - 210. [NPR3]
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 Home All anti-ARFGAP1 antibodies

Anti-ARFGAP1 TrueMAB Antibody clone 1F6

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

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Specifications Related Products Conjugation/Bulk FAQs
SKU Description Amount Price Availability*  
TA502593
  • Purified ARFGAP1 mouse monoclonal antibody, clone 1F6
  • FREE positive control: HEK293T cell transient overexpression lysate (LC406240) , 20ug
 100ul $325 In Stock Add to Shopping Cart
CF502593
  • Carrier-free (BSA/glycerol-free) ARFGAP1 mouse monoclonal antibody, clone 1F6
100ug $450 3-4 weeks Add to Shopping Cart
WB(2)
IHC(10)
IF(1)
FC(3)
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Also for ARFGAP1 (NM_175609)
cDNA Clone shRNA/siRNA Lysate Protein Antibody
Gene NameHomo sapiens ADP-ribosylation factor GTPase activating protein 1 (ARFGAP1), transcript variant 2
Synonyms:ARF1GAP; HRIHFB2281
ImmunogenFull length human recombinant protein of human ARFGAP1 (NP_783202) produced in HEK293T cell.
BufferPBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Clone Nameclone 1F6 IsotypeIgG2b
Species ReactivityHuman, Monkey Concentration0.5~1.0 mg/ml (Lot Dependent)
Purification Purified from mouse ascites fluids by affinity chromatography (Protein A or G Sepharose)
Guaranteed Application *WB, IHC, IF, FC Suggested DilutionsWB 1:1000~2000, IHC 1:150, IF 1:100, FLOW 1:100
BackgroundThe protein encoded by this gene is a GTPase-activating protein (GAP) which associates with the Golgi apparatus and which interacts with ADP-ribosylation factor 1 (ARF1). The encoded protein promotes hydrolysis of ARF1-bound GTP and is required for the dissociation of coat proteins from Golgi-derived membranes and vesicles. Dissociation of the coat proteins is required for the fusion of these vesicles with target compartments. The activity of this protein is stimulated by phosphoinosides and inhibited by phosphatidylcholine. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq].
Related Pathway

* Shipping is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ARFGAP1 (RC206987, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ARFGAP1.
WB Image
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-ARFGAP1 monoclonal antibody.
IHC Image
Immunohistochemical staining of paraffin-embedded Human Kidney tissue using anti-ARFGAP1 mouse monoclonal antibody. (TA502593)
IHC Image
Immunohistochemical staining of paraffin-embedded Human liver tissue using anti-ARFGAP1 mouse monoclonal antibody. (TA502593)
IHC Image
Immunohistochemical staining of paraffin-embedded Human pancreas tissue using anti-ARFGAP1 mouse monoclonal antibody. (TA502593)
IHC Image
Immunohistochemical staining of paraffin-embedded Human thyroid tissue using anti-ARFGAP1 mouse monoclonal antibody. (TA502593)
IHC Image
Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-ARFGAP1 mouse monoclonal antibody. (TA502593)
IHC Image
Immunohistochemical staining of paraffin-embedded Human endometrium tissue using anti-ARFGAP1 mouse monoclonal antibody. (TA502593)
IHC Image
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-ARFGAP1 mouse monoclonal antibody. (TA502593)
IHC Image
Immunohistochemical staining of paraffin-embedded Human prostate tissue using anti-ARFGAP1 mouse monoclonal antibody. (TA502593)
IHC Image
Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-ARFGAP1 mouse monoclonal antibody. (TA502593)
IHC Image
Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-ARFGAP1 mouse monoclonal antibody. (TA502593)
IF Image
Anti-ARFGAP1 mouse monoclonal antibody (TA502593) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY ARFGAP1(RC206987).
FC Image
HEK293T cells transfected with either RC206987 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-ARFGAP1 antibody(TA502593), and then analyzed by flow cytometry.
FC Image
Flow cytometric Analysis of Hela cells, using anti-ARFGAP1 antibody(TA502593),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).
FC Image
Flow cytometric Analysis of Jurkat cells, using anti-ARFGAP1 antibody(TA502593),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).

 

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