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Home Antibody All anti-ARFGAP1 antibodies

Anti-ARFGAP1 TRUEMAB Antibody clone 1H6

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA502531
  • ARFGAP1 mouse monoclonal antibody, clone 1H6
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC406240) , 20ug
100ul 325 In Stock
CF502531
  • Carrier-free (BSA/glycerol-free) ARFGAP1 mouse monoclonal antibody, clone 1H6
100ug $450 3-4 weeks
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WB(2)
IF(1)
FC(2)
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Also for ARFGAP1 (NM_175609)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenFull length human recombinant protein of human ARFGAP1 (NP_783202) produced in HEK293T cell.
Clone Nameclone 1H6 IsotypeIgG1
Species ReactivityHuman, Monkey Concentration0.60 mg/ml
Guaranteed Application *WB, IF, FC Suggested DilutionsWB 1:500~2000, IF 1:100, FLOW 1:100
BufferPBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from mouse ascites fluids by affinity chromatography

Reference Data

Target NameHomo sapiens ADP-ribosylation factor GTPase activating protein 1 (ARFGAP1), transcript variant 2
Alternative NameARF1GAP; HRIHFB2281
Database LinkNP_783202
FunctionThe protein encoded by this gene is a GTPase-activating protein (GAP) which associates with the Golgi apparatus and which interacts with ADP-ribosylation factor 1 (ARF1). The encoded protein promotes hydrolysis of ARF1-bound GTP and is required for the dissociation of coat proteins from Golgi-derived membranes and vesicles. Dissociation of the coat proteins is required for the fusion of these vesicles with target compartments. The activity of this protein is stimulated by phosphoinosides and inhibited by phosphatidylcholine. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq].
Related Pathway

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ARFGAP1 (RC206987, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ARFGAP1.
WB Image
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-ARFGAP1 monoclonal antibody.
IF Image
Anti-ARFGAP1 mouse monoclonal antibody (TA502531) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY ARFGAP1(RC206987).
FC Image
Flow cytometric Analysis of Hela cells, using anti-ARFGAP1 antibody(TA502531),(Red), compared to a nonspecific negative control antibody,(Blue).
FC Image
Flow cytometric Analysis of Jurkat cells, using anti-ARFGAP1 antibody(TA502531),(Red), compared to a nonspecific negative control antibody,(Blue).

 

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