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Anti-ARF1 Antibody EPR443
Also for ARF1 (NM_001658)
|A synthetic peptide corresponding to residues in human ARF1 was used as an immunogen.|
|Human, Mouse, Rat
||0.5~1.0 mg/ml (Lot Dependent)
||WB: 1:1000 - 1:10000; IHC-P: 1:100 - 1:250; ICC: 1:50 - 1:100
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant (Protein A or G Sepharose)
|Is unsuitable for Flow Cyt or IP.
|Homo sapiens ADP-ribosylation factor 1 (ARF1), transcript variant 4|
|OTTHUMP00000035715; ADP-ribosylation factor 1|
|ARF1 (small GTP-binding ADP-ribosylation factor 1) is a member of the ARF family, which contains five other ARF proteins (1). ARF1 functions in various membrane trafficking events in the ER-Golgi system and in the maintenance of organelle structure. Inactive ARF1 (ARF1-GDP) localizes in the cytoplasm, while the active form (ARF1-GTP) localizes in the membrane (2-3). Active ARF1 is also localized to the Golgi complex by regulating the assembly of coat proteins onto membranes and mediating traffic in the ER-Golgi. ARF1 activates phospholipase D and phosphatidylinositol 4-phosphate 5-kinase and recruits COPI to membranes (4-5).|
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Western blot - ARF1 antibody [EPR443]; All lanes : Anti-ARF1 antibody [EPR443] at 1/1000 dilution.Lane 1 : Human fetal kidney lysate.Lane 2 : Human fetal brain lysate.Lane 3 : MCF7 lysate.Lysates/proteins at 10 µg per lane.Predicted band size : 21 kDa.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - ARF1 antibody [EPR443]; Immunohistochemical analysis of ARF1 in paraffin embedded Human breast ductal carcinoma tissue, using ab108347 at a dilution of 1/100.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - ARF1 antibody [EPR443]; Immunohistochemical analysis of ARF1 in paraffin embedded Human colonic adenocarcinoma tissue, using ab108347 at a dilution of 1/100.