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Home Antibody All anti-AREG antibodies
UltraMAB

Anti-AREG GAT Antibody Polyclonal

Generated by Genomic Immunization

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Specifications Citations (0) Related Products Product Documents
SKU Description Amount Price Availability*  
TA590091
  • Rabbit Polyclonal Amphiregulin Antibody
100ug $325 In Stock
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WB(3)
IHC(9)
ELISA(1)

OriGene Data

ImmunogenDNA immunization. This antibody is specific for the N Terminus Region of the target protein.
Clone NamePolyclonal IsotypeIgG
Species ReactivityHuman , Dog , Monkey Concentration0.74mg/ml
Guaranteed Application *WB, ELISA, IHC Suggested Dilutions WB 1:5000~20000, IHC 1:150, ELISA 1:100-1:2000
Buffer20 mM Potassium Phosphate, 150 mM Sodium Chloride, pH 7.0
Purification The fragment used for DNA immunization was expressed in E.coli and the purified protein fragment was used for affinity purification of the antibody.
Note This antibody was generated by SDIX's Genomic Antibody Technology ® (GAT). Learn about GAT

Reference Data

Target NameHomo sapiens amphiregulin (AREG)
Alternative NameAR; AREGB; CRDGF; SDGF
Database LinkNP_001648
Entrez Gene 374 Human
FunctionThe protein encoded by this gene is a member of the epidermal growth factor family. It is an autocrine growth factor as well as a mitogen for astrocytes, Schwann cells, and fibroblasts. It is related to epidermal growth factor (EGF) and transforming growth factor alpha (TGF-alpha). This protein interacts with the EGF/TGF-alpha receptor to promote the growth of normal epithelial cells and inhibits the growth of certain aggressive carcinoma cell lines. This encoded protein is associated with a psoriasis-like skin phenotype.
Related PathwayTransmembraneDruggable Genome ErbB signaling pathway

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY AREG (RC203150, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-AREG.
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Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-AREG polyclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
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Western Blot: Amphiregulin Antibody - Western Blot was performed using affinity purified SEQer AR antibody, aa(88-187) antibody. The lanes contain 5-30ug of a whole cell extract. Final concentration of antibodies = 0.1ug/ml (1:10,000 dilution).
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ELISA: Amphiregulin Antibody - Affinity Purified
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Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-AREG Rabbit Polyclonal antibody. (TA590091)
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Immunohistochemical staining of paraffin-embedded Human Kidney tissue using anti-AREG Rabbit Polyclonal antibody. (TA590091)
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Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-AREG Rabbit Polyclonal antibody. (TA590091)
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Immunohistochemical staining of paraffin-embedded Human liver tissue using anti-AREG Rabbit Polyclonal antibody. (TA590091)
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Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-AREG Rabbit Polyclonal antibody. (TA590091)
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Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-AREG Rabbit Polyclonal antibody. (TA590091)
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Immunohistochemical staining of paraffin-embedded Human pancreas tissue using anti-AREG Rabbit Polyclonal antibody. (TA590091)
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Immunohistochemical staining of paraffin-embedded Human prostate tissue using anti-AREG Rabbit Polyclonal antibody. (TA590091)
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Immunohistochemical staining of paraffin-embedded Human lymph node tissue using anti-AREG Rabbit Polyclonal antibody. (TA590091)
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