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Anti-APEX1 TRUEMAB Antibody Clone OTI4D9

TrueMAB™ Antibodies - Made against Authentic Protein Antigens


Specifications Citations Customer Reviews Product Documents
SKU Description Amount Price Availability*  
TA800336 APEX1 mouse monoclonal antibody, clone OTI4D9 (formerly 4D9) 100ul 379
In Stock
LC400618 APEX1 HEK293T cell transient overexpression lysate (as WB positive control) 20ug $50 In Stock
CF800336 Carrier-free (BSA/glycerol-free) APEX1 mouse monoclonal antibody, clone OTI4D9 (formerly 4D9) 100ug $450 3 Days
Conjugation is available for this antibody: choose conjugation type
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Also for APEX1 (NM_001641)
cDNA Clone shRNA/siRNA CRISPR KO Kit Protein Antibody

OriGene Data

ImmunogenHuman recombinant protein fragment corresponding to amino acids 1-242 of human APEX1 (NP_001632) produced in E.coli.
Clone NameClone OTI4D9 IsotypeIgG2b
Species ReactivityHuman , Dog , Rat , Monkey , Mouse Concentration1 mg/ml
Guaranteed Application *WB, IHC Suggested Dilutions WB 1:2000, IHC 1:150,
Predicted MW Explanation 35.4 kDa
BufferPBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from mouse ascites fluids by affinity chromatography

Reference Data

Target NameHomo sapiens APEX nuclease (multifunctional DNA repair enzyme) 1 (APEX1), transcript variant 1
Alternative NameAPE; APE1; APEN; APEX; APX; HAP1; REF1
Database LinkNP_001632
Entrez Gene 328 Human
Entrez Gene 11792 Mouse
Entrez Gene 79116 Rat
Entrez Gene 702757 Monkey
Entrez Gene 482558 Dog
FunctionApurinic/apyrimidinic (AP) sites occur frequently in DNA molecules by spontaneous hydrolysis, by DNA damaging agents or by DNA glycosylases that remove specific abnormal bases. AP sites are pre-mutagenic lesions that can prevent normal DNA replication so the cell contains systems to identify and repair such sites. Class II AP endonucleases cleave the phosphodiester backbone 5' to the AP site. This gene encodes the major AP endonuclease in human cells. Splice variants have been found for this gene; all encode the same protein. [provided by RefSeq, Jul 2008].
Related PathwayStem cell - PluripotencyTranscription FactorsDruggable Genome Base excision repair

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY APEX1 (RC201732, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-APEX1.

WB Image
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-APEX1 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

IHC Image
Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-APEX1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA800336)


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