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Home Antibody All anti-APAF1 antibodies

Anti-APAF1 Antibody E38

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA303393
  • Rabbit monoclonal antibody against Apaf-1(E38)
100ul $325 3-7 Days
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WB(1)
IHC(1)
IF(1)

OriGene Data

ImmunogenA synthetic peptide corresponding to residues near N-terminus of human Apaf-1 was used as immunogen.
Clone NameE38 IsotypeRabbit IgG
Species ReactivityHuman, Mouse Concentration0.5~1.0 mg/ml (Lot Dependent)
Guaranteed Application *WB, IHC, IF Suggested DilutionsICC/IF: Use at an assay dependent concentration; WB: 1:1000 - 1:10000; IHC-P: 1:50 - 1:100; ICC: 1:50 - 1:100
BufferDoes not react with Rat. Is unsuitable for Flow Cyt or IP.
Purification PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Note Is unsuitable for Flow Cyt or IP.

Reference Data

Target NameHomo sapiens apoptotic peptidase activating factor 1 (APAF1), transcript variant 2
Alternative NameAPAF-1; CED4
Database LinkNP_001151
FunctionThis gene encodes a cytoplasmic protein that initiates apoptosis. This protein contains several copies of the WD-40 domain, a caspase recruitment domain (CARD), and an ATPase domain (NB-ARC). Upon binding cytochrome c and dATP, this protein forms an oligomeric apoptosome. The apoptosome binds and cleaves caspase 9 preproprotein, releasing its mature, activated form. Activated caspase 9 stimulates the subsequent caspase cascade that commits the cell to apoptosis. Alternative splicing results in several transcript variants encoding different isoforms. [provided by RefSeq].
Related Pathway
Apoptosis

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WB Image
Western blot - APAF1 antibody [E38]; Anti-APAF1 antibody [E38] at 1/500 dilution + HeLa cell lysate.Predicted band size : 141 kDa.Observed band size : 135 kDa .
IHC Image
Immunohistochemistry (Paraffin-embedded sections) - Cat# TA303393 - APAF1 antibody [E38] at a dilution of 1/50, staining APAF1 in paraffin embedded stomach adenocarcinoma by Immunohistochemistry.
IF Image
Immunocytochemistry/ Immunofluorescence - Anti-APAF1 antibody [E38]; TA303393 staining APAF1 in human leukocytes by Immunocytochemistry/ Immunofluorescence.Cells were fixed in formaldehyde, permeabilized using 0.3% Triton X-100, blocked with 2% serum for 2 hours at 25°C, and then incubated with TA303393 at a 1/250 dilution for 9 hours at 4°C. The secondary used was an Alexa-Fluor 488 conjugated goat anti-rabbit polyclonal used at a 1/500 dilution.

 

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