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Anti-APAF1 Antibody E38
Also for APAF1 (NM_001160)
|A synthetic peptide corresponding to residues near N-terminus of human Apaf-1 was used as immunogen.|
||0.5~1.0 mg/ml (Lot Dependent)
|WB, IHC, IF
||ICC/IF: Use at an assay dependent concentration; WB: 1:1000 - 1:10000; IHC-P: 1:50 - 1:100; ICC: 1:50 - 1:100
|Does not react with Rat. Is unsuitable for Flow Cyt or IP.|
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05% (Protein A or G Sepharose)
|Is unsuitable for Flow Cyt or IP.
|Homo sapiens apoptotic peptidase activating factor 1 (APAF1), transcript variant 2|
|This gene encodes a cytoplasmic protein that initiates apoptosis. This protein contains several copies of the WD-40 domain, a caspase recruitment domain (CARD), and an ATPase domain (NB-ARC). Upon binding cytochrome c and dATP, this protein forms an oligomeric apoptosome. The apoptosome binds and cleaves caspase 9 preproprotein, releasing its mature, activated form. Activated caspase 9 stimulates the subsequent caspase cascade that commits the cell to apoptosis. Alternative splicing results in several transcript variants encoding different isoforms. [provided by RefSeq]. |
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Western blot - APAF1 antibody [E38]; Anti-APAF1 antibody [E38] at 1/500 dilution + HeLa cell lysate.Predicted band size : 141 kDa.Observed band size : 135 kDa .
Immunohistochemistry (Paraffin-embedded sections) - APAF1 antibody [E38]; Ab32372, at a dilution of 1/50, staining APAF1 in paraffin embedded stomach adenocarcinoma by Immunohistochemistry.
Immunocytochemistry/ Immunofluorescence - Anti-APAF1 antibody [E38]; ab32372 staining APAF1 in human leukocytes by Immunocytochemistry/ Immunofluorescence.Cells were fixed in formaldehyde, permeabilized using 0.3% Triton X-100, blocked with 2% serum for 2 hours at 25°C, and then incubated with ab32372 at a 1/250 dilution for 9 hours at 4°C. The secondary used was an Alexa-Fluor 488 conjugated goat anti-rabbit polyclonal used at a 1/500 dilution.