Anti-AP2M1 Antibody, clone 1B11

Also for AP2M1 (NM_001025205)

Gene Name	Homo sapiens adaptor-related protein complex 2, mu 1 subunit (AP2M1), transcript variant 2
Synonyms:	AP50; CLAPM1; mu2
Immunogen	Human recombinant protein fragment corresponding to amino acids 97-383 of human AP2M1 (NP_001020376) produced in E.coli.
Buffer	PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Clone Name	clone 1B11	Isotype	IgG2a
Species Reactivity	Human, Monkey	Concentration	0.5~1.0 mg/ml (Lot Dependent)
Purification	Purified from mouse ascites fluids by affinity chromatography (Protein A or G Sepharose)
Guaranteed Application *	WB, IF, FC	Suggested Dilutions	WB 1:500~2000, IF 1:100, FLOW 1:100
Background	This gene encodes a subunit of the heterotetrameric coat assembly protein complex 2 (AP2), which belongs to the adaptor complexes medium subunits family. The encoded protein is required for the activity of a vacuolar ATPase, which is responsible for proton pumping occurring in the acidification of endosomes and lysosomes. The encoded protein may also play an important role in regulating the intracellular trafficking and function of CTLA-4 protein. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq].
Related Pathway

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WB Image

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY AP2M1 (RC201377, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-AP2M1.

WB Image

Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-AP2M1 monoclonal antibody.

IF Image

Immunofluorescent staining of HeLa cells using anti-AP2M1 mouse monoclonal antibody (TA503020).

FC Image

Flow cytometric Analysis of Hela cells, using anti-AP2M1 antibody(TA503020),(Red), compared to a nonspecific negative control antibody,(Blue).

FC Image

Flow cytometric Analysis of Jurkat cells, using anti-AP2M1 antibody(TA503020),(Red), compared to a nonspecific negative control antibody,(Blue).

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Anti-AP2M1 TrueMAB Antibody clone 1B11

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