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Home Antibody All anti-AP2A antibodies

Anti-AP2A Antibody

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA319408
  • Goat polyclonal anti-AP2A antibody
100ug 325 3-7 Days
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WB(1)

OriGene Data

ImmunogenThis affinity purified antibody was prepared from whole goat serum produced by repeated immunizations with a synthetic peptide corresponding aa 3-14 of Human AP2A1 and AP2A2 proteins.
Clone Name Isotype
Species Reactivityhuman, chimpanzee, orangutan, mouse, rat, chicken, dog Concentration1.7 mg/mL
Guaranteed Application *WB Suggested DilutionsELISA: 1:5,000 - 1:20,000, WB: 1:500 - 1:2,000, IP: 1:100
Buffer0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Note The AP2A1 and AP2A2 represent the AP2 alpha proteins that are found in the AP2 complex in clathrin-coated vesicles. AP2A is also known as Alpha-adaptin A, Adaptor protein complex AP-2 alpha-1 subunit, Clathrin assembly protein complex 2 alpha-A large chain, 100 kDa coated vesicle protein A and Plasma membrane adaptor HA2/AP2 adaptin alpha A subunit.  The AP2 complex is a heterotetramer consisting of two large adaptins (alpha or beta), a medium adaptin (mu), and a small adaptin (sigma). The complex is part of the protein coat on the cytoplasmic face of coated vesicles, which link clathrin to receptors in vesicles.

Reference Data

Target NameHomo sapiens adaptor-related protein complex 2, alpha 2 subunit (AP2A2), transcript variant 2
Alternative NameADTAB; CLAPA2; HIP-9; HIP9; HYPJ
Database LinkNP_036437
Function
Related Pathway

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot using Affinity Purified anti-AP2A antibody shows detection of a band just below 100 kDa correspond-ing to Human AP2A1 in a various preparations. Lane 1 - HeLa nuclear extract, Lane 2 - HeLa, Lane 3 - 293, Lane 4 - A431 and Lane 5 - Jurkat whole cell lysates. In lanes 6-10 the antibody was preincubated with 1 µg/ml of the immunizing peptide which effect-ively blocks the specific reactivity of this antibody with AP2A. Approximately 20 µg of each lysate was run on a SDS-PAGE and transferred onto nitrocellulose followed by reaction with a 1:500 dilution of anti-AP2A antibody. Detection occurred using a 1:5,000 dilution of HRP-labeled Rabbit anti-Goat IgG for 1 hour at room temperature. A chemi-luminescence system was used for signal detection (Roche) using a 60-sec exposure time.

 

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