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Anti-ANXA1 GAT Antibody Polyclonal
Generated by Genomic Immunization
Also for ANXA1 (NM_000700)
|DNA immunization. This antibody is specific for the Middle Region of the target protein.|
|Human , Dog , Rat , Monkey , Mouse
|WB, ELISA, IF
|| WB 1:5000~20000, IF 1:500,ELISA 1:100-1:2000
|20 mM Potassium Phosphate, 150 mM Sodium Chloride, pH 7.0|
|The fragment used for DNA immunization was expressed in E.coli and the purified protein fragment was used for affinity purification of the antibody.
|This antibody was generated by SDIX's Genomic Antibody Technology ® (GAT). Learn about GAT
|Homo sapiens annexin A1 (ANXA1)|
|Annexin I belongs to a family of Ca(2+)-dependent phospholipid binding proteins which have a molecular weight of approximately 35,000 to 40,000 and are preferentially located on the cytosolic face of the plasma membrane. Annexin I protein has an apparent relative molecular mass of 40 kDa, with phospholipase A2 inhibitory activity. Since phospholipase A2 is required for the biosynthesis of the potent mediators of inflammation, prostaglandins and leukotrienes, annexin I may have potential anti-inflammatory activity.|
Signaling by GPCR
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ANXA1 (RC201569, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ANXA1.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-ANXA1 polyclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
Immunofluorescent staining of HeLa cells using anti-ANXA1 rabbit polyclonal antibody (TA590778).