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Anti-ANKRD53 TRUEMAB Antibody clone 1E1
TrueMAB Antibodies - Made against Authentic Protein Antigens
Also for ANKRD53 (NM_024933)
|Human recombinant protein fragment corresponding to amino acids 1-300 of human ANKRD53 (NP_079209) produced in E.coli.|
|WB, IHC, IF, FC
||WB 1:500~2000, IHC 1:150, IF 1:100, FLOW 1:100
|PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.|
|Purified from mouse ascites fluids by affinity chromatography
|Homo sapiens ankyrin repeat domain 53 (ANKRD53), transcript variant 2|
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ANKRD53 (RC206701, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ANKRD53.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-ANKRD53 monoclonal antibody.
Immunohistochemical staining of paraffin-embedded Human lymph node tissue using anti-ANKRD53 mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA502601)
Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-ANKRD53 mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA502601)
Anti-ANKRD53 mouse monoclonal antibody (TA502601) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY ANKRD53(RC206701).
HEK293T cells transfected with either RC206701 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-ANKRD53 antibody(TA502601), and then analyzed by flow cytometry.
Flow cytometric Analysis of Hela cells, using anti-ANKRD53 antibody(TA502601),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).
Flow cytometric Analysis of Jurkat cells, using anti-ANKRD53 antibody(TA502601),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).