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Home Antibody All anti-ANKRD53 antibodies

Anti-ANKRD53 TRUEMAB Antibody Clone OTI1E1

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

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Specifications Citations (0) Related Products Product Documents
SKU Description Amount Price Availability*  
TA502601
  • ANKRD53 mouse monoclonal antibody, clone OTI1E1 (formerly 1E1)
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC410969) , 20ug Explanation
100ul $379 In Stock
CF502601
  • Carrier-free (BSA/glycerol-free) ANKRD53 mouse monoclonal antibody, clone OTI1E1 (formerly 1E1)
100ug $450 3-4 weeks

Buy any antibody of 100ul or more, get a free package of 3 loading control antibody samples. View Details Add to Shopping Cart

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WB(2)
IHC(2)
IF(1)
FC(3)

OriGene Data

ImmunogenHuman recombinant protein fragment corresponding to amino acids 1-300 of human ANKRD53 (NP_079209) produced in E.coli.
Clone NameClone OTI1E1 IsotypeIgG2b
Species ReactivityHuman Concentration0.58 mg/ml
Guaranteed Application *WB, IHC, IF, FC Suggested DilutionsWB 1:500~2000, IHC 1:150, IF 1:100, FLOW 1:100
Predicted MW Explanation 38 kDa
BufferPBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from mouse ascites fluids by affinity chromatography

Reference Data

Target NameHomo sapiens ankyrin repeat domain 53 (ANKRD53), transcript variant 2
Alternative NameFLJ12056; FLJ36160
Database LinkNP_079209
Entrez Gene 79998 Human
Function
Related Pathway

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ANKRD53 (RC206701, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ANKRD53.
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Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-ANKRD53 monoclonal antibody.
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Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-ANKRD53 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502601)
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Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-ANKRD53 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502601)
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Anti-ANKRD53 mouse monoclonal antibody (TA502601) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY ANKRD53(RC206701).
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HEK293T cells transfected with either RC206701 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-ANKRD53 antibody(TA502601), and then analyzed by flow cytometry.
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Flow cytometric Analysis of Hela cells, using anti-ANKRD53 antibody(TA502601),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).
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Flow cytometric Analysis of Jurkat cells, using anti-ANKRD53 antibody(TA502601),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).
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