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Home Antibody All anti-ANKRD53 antibodies

Anti-ANKRD53 TRUEMAB Antibody Clone OTI1B8

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

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Specifications Citations (0) Related Products Product Documents
SKU Description Amount Price Availability*  
TA502597 ANKRD53 mouse monoclonal antibody, clone OTI1B8 (formerly 1B8) 100ul $325 In Stock
LC410969 ANKRD53 HEK293T cell transient overexpression lysate (as WB positive control) 20ug $50 In Stock
CF502597 Carrier-free (BSA/glycerol-free) ANKRD53 mouse monoclonal antibody, clone OTI1B8 (formerly 1B8) 100ug $450 3-4 weeks
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WB(1)
IF(1)
FC(3)

OriGene Data

ImmunogenHuman recombinant protein fragment corresponding to amino acids 1-300 of human ANKRD53 (NP_079209) produced in E.coli.
Clone NameClone OTI1B8 IsotypeIgG1
Species ReactivityHuman Concentration0.66 mg/ml
Guaranteed Application *WB, IF, FC Suggested DilutionsWB 1:2000, IF 1:100, FLOW 1:100
Predicted MW Explanation 38 kDa
BufferPBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from mouse ascites fluids by affinity chromatography

Reference Data

Target NameHomo sapiens ankyrin repeat domain 53 (ANKRD53), transcript variant 2
Alternative NameFLJ12056; FLJ36160
Database LinkNP_079209
Entrez Gene 79998 Human
Function
Related Pathway

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ANKRD53 (RC206701, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ANKRD53.
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Anti-ANKRD53 mouse monoclonal antibody (TA502597) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY ANKRD53(RC206701).
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HEK293T cells transfected with either RC206701 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-ANKRD53 antibody(TA502597), and then analyzed by flow cytometry.
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Flow cytometric Analysis of Hela cells, using anti-ANKRD53 antibody(TA502597),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).
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Flow cytometric Analysis of Jurkat cells, using anti-ANKRD53 antibody(TA502597),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).
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