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Anti-AMFR GAT Antibody Polyclonal
Generated by Genomic Immunization
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Also for AMFR (NM_001144)
|DNA immunization. This antibody is specific for the C Terminus Region of the target protein.|
|Human , Rat , Monkey , Mouse
|WB, ELISA, IHC, IF
||WB 1:5000~20000, IHC 1:150, ELISA 1:100-1:2000
|20 mM Potassium Phosphate, 150 mM Sodium Chloride, pH 7.0|
|The fragment used for DNA immunization was expressed in E.coli and the purified protein fragment was used for affinity purification of the antibody.
|This antibody was generated by SDIX's Genomic Antibody Technology ® (GAT). Learn about GAT
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY AMFR (RC209639, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-AMFR.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-AMFR polyclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11; Lane 2: RT-4; Lane 3: U-251 MG; Lane 4: Human Plasma; Lane 5: Liver; Lane 6: TonsilThis validation was performed by Protein Atlas and the presentation of data is for informational purposes only.
Immunohistochemical staining of paraffin-embedded Human Kidney tissue using anti-AMFR Rabbit Polyclonal antibody. (TA590740)
Immunohistochemical staining of paraffin-embedded Human liver tissue using anti-AMFR Rabbit Polyclonal antibody. (TA590740)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-AMFR Rabbit Polyclonal antibody. (TA590740)
Immunohistochemical staining of human skeletal muscle shows strong cytoplasmic positivity in myocytes.This validation was performed by Protein Atlas and the presentation of data is for informational purposes only.
Immunofluorescent staining of human cell line U-251 MG shows no positivity.This validation was performed by Protein Atlas and the presentation of data is for informational purposes only.