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Home Antibody All anti-AMFR antibodies
UltraMAB

Anti-AMFR GAT Antibody Polyclonal

Generated by Genomic Immunization

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA590740
  • Rabbit Polyclonal AMFR Antibody
50ug 325 In Stock
This antibody qualifies for Season of Giving promotion: Earn $50 Amazon gift card (use promo code SPQ12081) or donation to Cancer.org (promo code SPQ12082) with $600 purchase of UltraMAB or TrueMAB. Click for details
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WB(3)
IHC(4)
IF(1)
ELISA(1)

OriGene Data

ImmunogenDNA immunization. This antibody is specific for the C Terminus Region of the target protein.
Clone NamePolyclonal IsotypeIgG
Species ReactivityHuman , Rat , Monkey , Mouse Concentration0.8mg/ml
Guaranteed Application *WB, ELISA, IHC, IF Suggested Dilutions WB 1:5000~20000, IHC 1:150, ELISA 1:100-1:2000
Buffer20 mM Potassium Phosphate, 150 mM Sodium Chloride, pH 7.0
Purification The fragment used for DNA immunization was expressed in E.coli and the purified protein fragment was used for affinity purification of the antibody.
Note This antibody was generated by SDIX's Genomic Antibody Technology ® (GAT). Learn about GAT

Reference Data

Target NameHomo sapiens autocrine motility factor receptor, E3 ubiquitin protein ligase (AMFR)
Alternative NameGP78; RNF45
Database LinkNP_001135
Entrez Gene 267 Human
Entrez Gene 23802 Mouse
Entrez Gene 361367 Rat
Entrez Gene 699972 Monkey
FunctionAutocrine motility factor is a tumor motility-stimulating protein secreted by tumor cells. The protein encoded by this gene is a glycosylated transmembrane protein and a receptor for autocrine motility factor. The receptor, which shows some sequence similarity to tumor protein p53, is localized to the leading and trailing edges of carcinoma cells.
Related PathwayTransmembraneDruggable Genome

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY AMFR (RC209639, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-AMFR.
WB Image
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-AMFR polyclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
WB Image
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11; Lane 2: RT-4; Lane 3: U-251 MG; Lane 4: Human Plasma; Lane 5: Liver; Lane 6: TonsilThis validation was performed by Protein Atlas and the presentation of data is for informational purposes only.
ELISA Image
ELISA: AMFR Antibody
IHC Image
Immunohistochemical staining of paraffin-embedded Human Kidney tissue using anti-AMFR Rabbit Polyclonal antibody. (TA590740)
IHC Image
Immunohistochemical staining of paraffin-embedded Human liver tissue using anti-AMFR Rabbit Polyclonal antibody. (TA590740)
IHC Image
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-AMFR Rabbit Polyclonal antibody. (TA590740)
IHC Image
Immunohistochemical staining of human skeletal muscle shows strong cytoplasmic positivity in myocytes.This validation was performed by Protein Atlas and the presentation of data is for informational purposes only.
IF Image
Immunofluorescent staining of human cell line U-251 MG shows no positivity.This validation was performed by Protein Atlas and the presentation of data is for informational purposes only.

 

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