EGFR Mouse Monoclonal Antibody [Clone ID: H9B4]

CAT#: AM50266PU-S

EGFR mouse monoclonal antibody, clone H9B4, Purified

Size: 20 ug 100 ug


USD 504.00

2 Weeks*

Size
    • 100 ug

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Specifications

Product Data
Clone Name H9B4
Applications IP, WB
Recommended Dilution ELISA: Use BSA free Antibody for coating.
Immunoprecipitation: 1-2 µg/500ug protein lysate. 
Western Blotting: 0.5-1.0 µg/ml for 2 hours at RT.
Positive Control: A431 cells, Breast or Bladder cancer.
Reactivities Human
Host Mouse
Isotype IgG1
Clonality Monoclonal
Immunogen Purified EGFR from A431 cells.
Specificity This MAb reacts with a cytoplasmic domain of EGFR. EGFR is a type I receptor tyrosine kinase with sequence homology to erbB-1, -2, -3 -4 or HER-1, -2, -3 -4. It binds to Epidermal Growth Factor (EGF), Transforming Growth Factor-a (TGF-a), Heparin-binding EGF (HB-EGF), amphiregulin, betacellulin & epiregulin.
Cellular Localization: Cell surface.
Formulation 10mM PBS
State: Purified
State: Liquid purified IgG fraction from Bioreactor Concentrate
Stabilizer: 0.05% BSA
Preservative: 0.05% Sodium Azide
Concentration lot specific
Purification Protein A/G Chromatography
Conjugation Unconjugated
Storage Store undiluted at 2-8°C.
Stability Shelf life: one year from despatch.
Predicted Protein Size ~170 kDa (wild type) and ~145 kDa (vIII variant)
Background EGFR is type I receptor tyrosine kinase with sequence homology to erbB-1, -2, -3 -4 or HER-1, -2, -3 -4. It binds to Epidermal Growth Factor (EGF), Transforming Growth Factor-a (TGF-a), Heparin-binding EGF (HB-EGF), amphiregulin, betacellulin and epiregulin. EGFR is overexpressed in tumors of breast, brain, bladder, lung, gastric, head & neck, esophagus, cervix, vulva, ovary, and endometrium. It is predominantly present in squamous cell carcinomas.
Synonyms Epidermal growth factor receptor, EGF Receptor, erbB-1, c-ErbB-1
Reference Data

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.