EGFR Mouse Monoclonal Antibody [Clone ID: GFR450]

CAT#: AM32821PU-T

EGFR mouse monoclonal antibody, clone GFR450, Purified

Size: 20 ug 100 ug


USD 293.00

2 Weeks*

Size
    • 20 ug

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Specifications

Product Data
Clone Name GFR450
Applications FC, IF, IHC
Recommended Dilution Immunohistochemistry on Paraffin Sections: 0.5-1 µg/ml for 30 minutes at RT.
Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes. 
Positive Control: A431 cells, Breast or Bladder cancer. 
Flow Cytometry: 0.5-1 µg/million cells.
Immunofluorescence: 1-2 µg/ml.
Reactivities Human
Host Mouse
Isotype IgG2a
Clonality Monoclonal
Immunogen Recombinant Human EGFR protein
Specificity This Monoclonal antibody GFR450 recognizes a protein of 170kDa, identified as EGFR. 
Cellular Localization: Cell surface.
Negative Species: Mouse.
Formulation 10mM PBS
State: Purified
State: Liquid purified IgG fraction from Bioreactor Concentrate
Stabilizer: 0.05% BSA
Preservative: 0.05% Sodium Azide
Concentration lot specific
Purification Protein A/G Chromatography
Conjugation Unconjugated
Storage Store undiluted at 2-8°C.
Stability Shelf life: one year from despatch.
Predicted Protein Size ~170 kDa (wild type) and ~145 kDa (vIII variant)
Background EGFR is type I receptor tyrosine kinase with sequence homology to erbB-1, -2, -3 -4 or HER-1, -2, -3 -4. It binds to Epidermal Growth Factor (EGF), Transforming Growth Factor-a (TGF-a), Heparin-binding EGF (HB-EGF), amphiregulin, betacellulin and epiregulin. EGFR is overexpressed in tumors of breast, brain, bladder, lung, gastric, head & neck, esophagus, cervix, vulva, ovary, and endometrium. It is predominantly present in squamous cell carcinomas.
Synonyms Epidermal growth factor receptor, EGF Receptor, erbB-1, c-ErbB-1
Reference Data

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.