EGFR Mouse Monoclonal Antibody [Clone ID: GFR450]
Other products for "EGFR"
Specifications
Product Data | |
Clone Name | GFR450 |
Applications | FC, IF, IHC |
Recommended Dilution | Immunohistochemistry on Paraffin Sections: 0.5-1 µg/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes. Positive Control: A431 cells, Breast or Bladder cancer. Flow Cytometry: 0.5-1 µg/million cells. Immunofluorescence: 1-2 µg/ml. |
Reactivities | Human |
Host | Mouse |
Isotype | IgG2a |
Clonality | Monoclonal |
Immunogen | Recombinant Human EGFR protein |
Specificity | This Monoclonal antibody GFR450 recognizes a protein of 170kDa, identified as EGFR. Cellular Localization: Cell surface. Negative Species: Mouse. |
Formulation | 10mM PBS State: Purified State: Liquid purified IgG fraction from Bioreactor Concentrate Stabilizer: 0.05% BSA Preservative: 0.05% Sodium Azide |
Concentration | lot specific |
Purification | Protein A/G Chromatography |
Conjugation | Unconjugated |
Storage | Store undiluted at 2-8°C. |
Stability | Shelf life: one year from despatch. |
Predicted Protein Size | ~170 kDa (wild type) and ~145 kDa (vIII variant) |
Database Link | |
Background | EGFR is type I receptor tyrosine kinase with sequence homology to erbB-1, -2, -3 -4 or HER-1, -2, -3 -4. It binds to Epidermal Growth Factor (EGF), Transforming Growth Factor-a (TGF-a), Heparin-binding EGF (HB-EGF), amphiregulin, betacellulin and epiregulin. EGFR is overexpressed in tumors of breast, brain, bladder, lung, gastric, head & neck, esophagus, cervix, vulva, ovary, and endometrium. It is predominantly present in squamous cell carcinomas. |
Synonyms | Epidermal growth factor receptor, EGF Receptor, erbB-1, c-ErbB-1 |
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