VEGFA Mouse Monoclonal Antibody [Clone ID: 4B2-8]

CAT#: AM01371PU-N

VEGFA mouse monoclonal antibody, clone 4B2-8, Azide Free

Specifications

Product Data

• Clone Name: 4B2-8
• Applications: ELISA, WB
• Recommended Dilution: ELISA: In a Sandwich ELISA (assuming 100ul/well), a concentration of 2.0-4.0 μg/ml of this antibody will detect at least 100 pg/ml of recombinant Human VEGF165 when used with Biotin anti-Human VEGF165 antibody (Cat.-No PP1073B) as the detection antibody at a concentration of approximately 0.25-1.0 μg/ml.
  Western Blot: To detect Human VEGF165 by Western Blot analysis this antibody can be used at a concentration of 0.25-0.50 μg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant Human VEGF165 is 0.25-0.50 ng/lane, under non-reducing conditions.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Highly pure (>98%) E.coli derived recombinant Human VEGF165 (Cat.-No PA136).
• Specificity: This antibody detects Vascular Endothelial Growth Factor. Other species not tested.
• Formulation: PBS without preservatives
  State: Azide Free
  State: Lyophilized (sterile filtered) purified IgG fraction from cell culture
• Reconstitution Method: Restore in sterile water to a concentration of 1.0 mg/ml.
• Purification: Affinity Chromatography on Protein A
• Conjugation: Unconjugated
• Storage: Store lyophilized at 2-8°C for 6 months or at -20°C long term.
  After reconstitution store the antibody undiluted at 2-8°C for one month
  or (in aliquots) at -20°C long term.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: vascular endothelial growth factor A
• Database Link:
  Entrez Gene 7422 Human
  UniProt ID P15692
• Synonyms: VEGFA, VEGF, VPF, Vascular endothelial growth factor A, Vascular permeability factor