EGFR (N-term) (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 14C8]

CAT#: AM00045BT-N

EGFR (N-term) (incl. pos. control) mouse monoclonal antibody, clone 14C8, Biotin

Conjugation: Unconjugated Biotin


USD 543.00

2 Weeks*

Size
    • 100 ug

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Specifications

Product Data
Clone Name 14C8
Applications WB
Recommended Dilution Western Blot: 0.5 µg/ml for HRPO/ECL detection.
Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubation buffer. 
Included Positive Control: Cell lysate from untreated HepG2 cells (See Protocols).
Reactivities Canine, Human, Mouse
Host Mouse
Isotype IgG1
Clonality Monoclonal
Immunogen Peptide conjugated to KLH. 
Epitope: N-terminus.
Specificity This antibody specifically recognizes the N-terminus of EGF receptor.
Formulation PBS
Label: Biotin
State: Liquid purified Ig fraction from Serum-free Cell Culture Supernatant
Stabilizer: PEG and Sucrose
Preservative: 0.09% Sodium Azide
Concentration lot specific
Purification Size Exclusion Chromatography
Conjugation Biotin
Storage Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Stability Shelf life: one year from despatch.
Predicted Protein Size 180 kDa
Background EGF Receptor (EGFR) and erbB2, erbB3, and ErbB4 are members of subclass I of receptor tyrosine kinases. EGFR/erbB receptors are activated upon binding of EGF and EGF-related growth factors such as TGF alpha, beta-cellulin, Hb-EGF, HRG, or NRG. Binding of these ligands leads to receptor homo- and heterodimerization followed by autophosphorylation and activation of downstream signal transduction pathways (MAPK, PI3K/PKB, and STAT). In addition, EGFR becomes fully activated after phosphorylation of Y845 by src family kinases. Phosphorylation of Y1045 leads to association with cbl and subsequent receptor degradation. Phosphorylation of S1047 by CamKinase II leads to attenuation of kinase activity; phosphorylation of T654 (by PKC) and T669 (by MAPK, p38) interferes with receptor endocytosis/recycling.
Synonyms Epidermal growth factor receptor, EGF Receptor, erbB-1, c-ErbB-1
Note Protocol: Included Positive Control: Cell Lysate from untreated HepG2 cells.

Formulation: Lyophilized cell lysate from serum starved HepG2 cells.

Reconstitution: Restore by addition of 200 µl H2O. After complete solubilization add 200 µl 2x SDS-PAGE sample buffer, mix and incubate at 90°C for 5 min.

Storage
: Aliquote and store frozen. Avoid repeated freeze/thaw cycles.

Application
: The postiive control cell lysate is recommended for immunoblot applications. 20 µl of positive control cell lysate correspond to ca. 80.000 cells.
Use 20 µl/lane (mini gel) for HRPO/ECL detection of the target proteins.

Please Note: The lyophilized cell lysates contain SDS and are not recommended for applications with native proteins such as immunoprecipitation.
Reference Data

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.