EGFR pTyr1173 Mouse Monoclonal Antibody [Clone ID: 9H2]

CAT#: AM00042FC-N

EGFR pTyr1173 mouse monoclonal antibody, clone 9H2, FITC

Conjugation: Unconjugated Biotin FITC


USD 618.00

2 Weeks*

Size
    • 100 ug

Product Images

Other products for "EGFR"

Specifications

Product Data
Clone Name 9H2
Applications IF
Recommended Dilution Immunocytochemistry: Use at 1-10 μg/ml.
For ELISA, Immunoprecipitation and Immunoblotting, use Purified antibody (Cat.-No. AM00042PU-N) or Biotinylated antibody (Cat.-No. AM00042BT-N).
Reactivities Canine, Human, Mouse
Host Mouse
Isotype IgG1
Clonality Monoclonal
Immunogen Phosphopeptide conjugated to KLH.
Epitope: Phospho-Tyr1197 N A E pY L R V
Specificity This antibody specifically interacts with the 1197 - N A E pY L R V motif corresponding to the major autophosphorylation site of Human EGFR.
Antibody AM00042FC-N does not crossreact with the highly homologous pTyr1248 of acticated erbB2.
Formulation PBS containing 0.09% Sodium Azide/PEG and Sucrose.
Label: FITC
State: Liquid purified Ig fraction.
Concentration lot specific
Purification Purified from serum-free cell culture supernatant by Subsequent Thiophilic Adsorption and Size Exclusion Chromatography.
Conjugation FITC
Storage Store the antibody (aliquote in liquid nitrogen) at -80°C.
Avoid repeated freezing and thawing.
Thaw aliquots at 37°C. Thawed aliquots may be stored at 4°C up to 3 months.
Stability Shelf life: one year from despatch.
Background EGFR/erbB receptors are activated upon binding of EGF and EGF-related growth factors such as TGF alpha, beta-cellulin, Hb-EGF, HRG, or NRG. Binding of these ligands leads to receptor homo- and heterodimerization followed by autophosphorylation and activation of downstream signal transduction pathways (MAPK, PI3K/PKB, and STAT). In addition, EGFR becomes fully activated after phosphorylation of Y869 by src family kinases.
Phosphorylation of Y1069 leads to association with cbl and subsequent receptor degradation. Phosphorylation of S1071 by CamKinase II leads to attenuation of kinase activity; phosphorylation of T678 (by PKC) and T693 (by MAPK, p38) interferes with receptor endocytosis/recycling.
Synonyms Epidermal growth factor receptor, EGF Receptor, erbB-1, c-ErbB-1
Reference Data

{0} Product Review(s)

0 Product Review(s) Submit review

Be the first one to submit a review

Product Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.