EGFR pTyr1172 (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 10G12]

CAT#: AM00041PU-N

EGFR pTyr1172 (incl. pos. control) mouse monoclonal antibody, clone 10G12, Purified


USD 543.00

2 Weeks*

Size
    • 100 ug

Product Images

Other products for "EGFR"

Specifications

Product Data
Clone Name 10G12
Applications WB
Recommended Dilution Western blot (0.5 µg/ml for HRPO/ECL detection; recommended blocking buffer: Casein/Tween 20 based blocking and blot incubation buffer).
Use cell lysate from EGF-treated HepG2 cells as positive control.
Reactivities Human
Host Mouse
Isotype IgG
Clonality Monoclonal
Immunogen Phosphopeptide conjugated to hemocyanin
N P D pY Q Q D
Specificity This antibody specifically recognizes EGFR phosphorylated at Tyrosine 1172.
Formulation PBS, 0.09 % Na-azide, PEG and Sucrose
State: Purified
State: Lyophilized Ig fraction
Reconstitution Method Reconstitute with 1 ml H2O (15 min, RT).
Purification Subsequent ultrafiltration and size exclusion
Conjugation Unconjugated
Storage Aliquot and store at -20 °C. Avoid repeated freezing and thawing.
Stability Shelf life: One year from despatch.
Background EGFR/erbB receptors are activated upon binding of EGF and EGF-related growth factors such as TGF alpha, beta-cellulin, Hb-EGF, HRG, or NRG. Binding of these ligands leads to receptor homo- and heterodimerization followed by autophosphorylation and activation of downstream signal transduction pathways (MAPK, PI3K/PKB, and STAT). In addition, EGFR becomes fully activated after phosphorylation of Y869 by src family kinases.
Phosphorylation of Y1069 leads to association with cbl and subsequent receptor degradation. Phosphorylation of S1071 by CamKinase II leads to attenuation of kinase activity; phosphorylation of T678 (by PKC) and T693 (by MAPK, p38) interferes with receptor endocytosis/recycling.
Synonyms Epidermal growth factor receptor, EGF Receptor, erbB-1, c-ErbB-1
Note Mol. weight: 180 kDa
A positive control is provided (for details see protocol below).

Protocol: Positive control cell lysate provided:
HepG2 EGF Treated - recommended for immunoblotting

Formulation:
Lyophilized cell lysate from HepG2 cells. Serum starved cells were treated for 15 min. with EGF.
Restore by addition of 200 µl H2O. After complete solubilization add 200 µl 2x SDS-PAGE sample buffer, mix and incubate at 90°C for 5 min. Store in aliquots at -20°C. Avoid repeated freezing and thawing.

Applications:
20 µl of positive cell lysate correspond to ca. 80.000 cells. Use 20 µl / lane (mini gel) for HRPO/ECL detection of the target proteins.

Please note:
The lyophilized cell lysate contains SDS and is not recommended for applications with native proteins such as immunoprecipitation.
Reference Data

{0} Product Review(s)

0 Product Review(s) Submit review

Be the first one to submit a review

Product Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.