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Anti-ALPL Antibody EPR4477
Also for ALPL (NM_000478)
|A synthetic peptide corresponding to residues in human ALPL was used as an immunogen.|
|Human, Mouse, Rat
||0.5~1.0 mg/ml (Lot Dependent)
|WB, IHC, IF, FC
||FC: 1:1000; WB: 1:10000 - 1:50000; IP: 1:10 - 1:100; IHC-P: 1:250 - 1:500; ICC: 1:100 - 1:500
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant (Protein A or G Sepharose)
|Homo sapiens alkaline phosphatase, liver/bone/kidney (ALPL), transcript variant 1|
|AP-TNAP; APTNAP; HOPS; TNAP; TNSALP|
|There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue non-specific). The first three are located together on chromosome 2, while the tissue non-specific form is located on chromosome 1. ALPL is a membrane bound glycosylated enzyme that is not expressed in any particular tissue and is, therefore, referred to as the tissue-nonspecific form of the enzyme. The exact physiological function of the alkaline phosphatases is not known. A proposed function of this form of the enzyme is matrix mineralization; however, mice that lack a functional form of this enzyme show normal skeletal development. This enzyme has been linked directly to hypophosphatasia, a disorder that is characterized by hypercalcemia and includes skeletal defects. The character of this disorder can vary, however, depending on the specific mutation since this determines age of onset and severity of symptoms (1).|
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Western blot - Bone Alkaline Phosphatase antibody [EPR4477]; All lanes : Anti-Alkaline Phosphatase, Tissue Non-Specific [EPR4477] antibody at 1/10000 dilution.Lane 1 : SAOS2 cell lysate.Lane 2 : HepG2 cell lysate.Lane 3 : HeLa cell lysate.Lane 4 : JAR cell lysate.Lysates/proteins at 10 µg per lane.Predicted band size : 57 kDa.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Bone Alkaline Phosphatase antibody [EPR4477]; ab108337, at a 1/250 dilution, staining Bone Alkaline Phosphatase in paraffin embedded Human kidney tissue by Immunohistochemistry.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Bone Alkaline Phosphatase antibody [EPR4477]; ab108337, at a 1/250 dilution, staining Bone Alkaline Phosphatase in paraffin embedded Human liver tissue by Immunohistochemistry.
Immunocytochemistry/ Immunofluorescence - Bone Alkaline Phosphatase antibody [EPR4477]; ab108337, at a 1/100 dilution, staining Bone Alkaline Phosphatase in HeLa cells by Immunofluorescence.
Flow Cytometry - Anti-Alkaline Phosphatase, Tissue Non-Specific [EPR4477] antibody; Overlay histogram showing Saos 2 cells stained with ab108337 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was DyLight? 488 goat anti-rabbit IgG (H+L) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1µg/1x10^6 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in Saos 2 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.