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Home Antibody All anti-ALOX15 antibodies

Anti-ALOX15 TRUEMAB Antibody Clone OTI7H6

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

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Specifications Citations (3) Related Products Product Documents
SKU Description Amount Price Availability*  
TA504358 ALOX15 mouse monoclonal antibody, clone OTI7H6 (formerly 7H6) 100ul $325 In Stock
LC400456 ALOX15 HEK293T cell transient overexpression lysate (as WB positive control) 20ug $50 In Stock
CF504358 Carrier-free (BSA/glycerol-free) ALOX15 mouse monoclonal antibody, clone OTI7H6 (formerly 7H6) 100ug $450 3-4 weeks
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WB(2)
IHC(1)
IF(1)
FC(1)
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Also for ALOX15 (NM_001140)
cDNA Clone shRNA/siRNA CRISPR KO Kit Protein Antibody

OriGene Data

ImmunogenFull length human recombinant protein of human ALOX15(NP_001131) produced in HEK293T cell.
Clone NameClone OTI7H6 IsotypeIgG2b
Species ReactivityHuman Concentration1.21 mg/ml
Guaranteed Application *WB, IHC, IF, FC Suggested DilutionsWB 1:2000, IHC 1:150, IF 1:100, FLOW 1:100,
Predicted MW Explanation 74.6 kDa
BufferPBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from mouse ascites fluids by affinity chromatography

Reference Data

Target NameHomo sapiens arachidonate 15-lipoxygenase (ALOX15)
Alternative Name12-LOX; 15-LOX-1; 15LOX-1
Database LinkNP_001131
Entrez Gene 246 Human
Function
Related PathwayDruggable Genome Arachidonic acid metabolismLinoleic acid metabolismMetabolic pathways

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ALOX15 (RC206621, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ALOX15.
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Western blot analysis of extracts (10ug) from 2 different cell lines by using anti-ALOX15 monoclonal antibody.(1:200)
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Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-ALOX15 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504358)
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Anti-ALOX15 mouse monoclonal antibody (TA504358) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY ALOX15(RC206621).
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HEK293T cells transfected with either RC206621 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-ALOX15 antibody(TA504358), and then analyzed by flow cytometry.
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