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Anti-ALK TRUEMAB Antibody Clone OTI1A4

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

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Specifications Citations (2) Related Products Product Documents
SKU Description Amount Price Availability*  
TA801287 ALK mouse monoclonal antibody, clone OTI1A4 (formerly 1A4) 100ul $379 In Stock
LC418072 ALK HEK293T cell transient overexpression lysate (as WB positive control) 20ug $50 In Stock
CF801287 Carrier-free (BSA/glycerol-free) ALK mouse monoclonal antibody, clone OTI1A4 (formerly 1A4) 100ug $450 3 Days
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WB(2)
IHC(7)

OriGene Data

ImmunogenHuman recombinant protein fragment corresponding to amino acids 1300-1620 of human ALK (NP_004295) produced in E.coli.
Clone NameClone OTI1A4 IsotypeIgG2b
Species ReactivityHuman Concentration1 mg/ml
Guaranteed Application *WB, IHC Suggested Dilutions WB 1:2000,
Predicted MW Explanation 176.3 kDa
BufferPBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from mouse ascites fluids by affinity chromatography

Reference Data

Target NameHomo sapiens anaplastic lymphoma receptor tyrosine kinase (ALK)
Alternative NameCD246; NBLST3
Database LinkNP_004295
Entrez Gene 238 Human
FunctionThis gene encodes a receptor tyrosine kinase, which belongs to the insulin receptor superfamily. This protein comprises an extracellular domain, an hydrophobic stretch corresponding to a single pass transmembrane region, and an intracellular kinase domain. It plays an important role in the development of the brain and exerts its effects on specific neurons in the nervous system. This gene has been found to be rearranged, mutated, or amplified in a series of tumours including anaplastic large cell lymphomas, neuroblastoma, and non-small cell lung cancer. The chromosomal rearrangements are the most common genetic alterations in this gene, which result in creation of multiple fusion genes in tumourigenesis, including ALK (chromosome 2)/EML4 (chromosome 2), ALK/RANBP2 (chromosome 2), ALK/ATIC (chromosome 2), ALK/TFG (chromosome 3), ALK/NPM1 (chromosome 5), ALK/SQSTM1 (chromosome 5), ALK/KIF5B (chromosome 10), ALK/CLTC (chromosome 17), ALK/TPM4 (chromosome 19), and ALK/MSN (chromosome X).[provided by RefSeq, Jan 2011].
Related PathwayProtein KinaseDruggable Genome

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ALK (RC222485, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ALK.
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Western blot analysis of extracts (35ug) from H2228 and A549 cell lines by using anti-ALK monoclonal antibody. (TA801287, 1:10,000)
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Immunohistochemical staining of paraffin-embedded Human non-small cell lung cancer sample with ALK translocation detected by FISH  using anti-ALK mouse monoclonal antibody. (TA801287, 1:50; heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.0, 120C for 3min)
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Immunohistochemical staining of paraffin-embedded Human large B cell lymphoma with ALK translocation  using anti-ALK mouse monoclonal antibody. (TA801287, 1:50; heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.0, 120C for 3min)
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Immunohistochemical staining of paraffin-embedded ALK-positive lung tumor xenograft  using anti-ALK mouse monoclonal antibody. (TA801287, 1:50; heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.0, 120C for 3min)
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Immunohistochemical staining of paraffin-embedded Human non-small cell lung cancer sample with EML4-ALK translocation detected by PCR  using anti-ALK mouse monoclonal antibody. (TA801287, 1:50; heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.0, 120C for 3min)
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Immunohistochemistry staining of paraffin-embedded human cell line H1975 (upper left) and H2228 (upper right) on IHC antibody quality control slide using anti-ALK mouse monoclonal antibody TA801287 (1:400). The ALK rearrangement in H2228 cells is labeled with ALK Breakapart probe in FISH test (lower right, 60X) and the control of H1975 cell at the same FISH probe test (lower left, 60X).
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Immunohistochemical staining of paraffin-embedded human ALK-positive lung cancer tissue using anti-ALK mouse monoclonal antibody. (TA801287, 1:100 for 30 min at RT; heat-induced epitope retrieval by TEE, pH9.0)
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Figure from citation: Immunohistochemistry of ALK protein level by using anti-ALK antibody in human right cervical lymph node. Dilution: 1:200 View Citation
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