Home Antibody All anti-ALDH1A1 antibodies
Anti-ALDH1A1 GAT Antibody Polyclonal
Generated by Genomic Immunization
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Also for ALDH1A1 (NM_000689)
|DNA immunization. This ALDH1A1 antibody is specific for the N Terminus Region of the human ALDH1A1 protein.|
|WB, ELISA, IHC, IF
|| WB 1:5000~20000,ELISA 1:100-1:2000
|20 mM Potassium Phosphate, 150 mM Sodium Chloride, pH 7.0|
|The fragment used for DNA immunization was expressed in E.coli and the purified protein fragment was used for affinity purification of the antibody.
|This antibody was generated by SDIX's Genomic Antibody Technology ® (GAT). Learn about GAT
|Homo sapiens aldehyde dehydrogenase 1 family, member A1 (ALDH1A1)|
|ALDC; ALDH-E1; ALDH1; ALDH11; HEL-9; HEL-S-53e; HEL12; PUMB1; RALDH1|
Entrez Gene 216 Human
|Usual human livers contain two major ALDH isozymes, i.e., cytosolic ALDH1 and mitochondrial ALDH2(1). Human red cell aldehyde dehydrogenase (ALDH) resembles the liver cytosolic isozyme in numerous physicochemical properties. Thus, the erythrocyte enzyme, by virtue of its chemical and structural identity with the liver cytosolic enzyme, may serve as a suitable peripheral enzyme model to understand the cause and mechanism of alcohol abuse-related changes in liver cytosolic ALDH that has been found to be reduced in alcoholics (2). ALDH1A1 is known to catalyze the oxidation of retinaldehyde to retinoic acid. Overexpression of human ALDH1A1 in HEK cells led to a more than 20-fold increase in 3-deoxyglucosone dehydrogenase activity (3).|
|ES Cell Differentiation/IPSDruggable Genome Retinol metabolismMetabolic pathways|
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ALDH1A1 (RC200723, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ALDH1A1.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-ALDH1A1 polyclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11; Lane 2: RT-4; Lane 3: U-251 MG; Lane 4: Human Plasma; Lane 5: Liver; Lane 6: TonsilThis validation was performed by Protein Atlas and the presentation of data is for informational purposes only.
Immunohistochemical staining of human stomach shows strong positivity in Parietal cells.This validation was performed by Protein Atlas and the presentation of data is for informational purposes only.
Immunocytochemistry/Immunofluorescence: ALDH1A1 Antibody - Detection of ALDH1A1 (Green) in HepG2 cells using 31160002. Nuclei (Blue) are counterstained using Hoechst 33258.
Immunofluorescent staining of human cell line A-431 shows no positivity.This validation was performed by Protein Atlas and the presentation of data is for informational purposes only.