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Anti-ALDH1A1 Antibody EP1933Y
Also for ALDH1A1 (NM_000689)
|A synthetic peptide corresponding to residues near the C-terminus of human Aldh1a1 was used as an immunogen.|
|Human, Mouse, Rat
||0.5~1.0 mg/ml (Lot Dependent)
|WB, IHC, IF
||ICC/IF: Use at an assay dependent concentration; WB: 1:500 - 1:2000; IP: 1:30; ICC: 1:100 - 1:250; IHC-P: Use at an assay dependent dilution
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant (Protein A or G Sepharose)
|Is unsuitable for Flow Cyt.
|Homo sapiens aldehyde dehydrogenase 1 family, member A1 (ALDH1A1)|
|ALDC; ALDH-E1; ALDH1; ALDH11; PUMB1; RALDH1|
|This protein belongs to the aldehyde dehydrogenases family of proteins. Aldehyde dehydrogenase is the second enzyme of the major oxidative pathway of alcohol metabolism. Two major liver isoforms of this enzyme, cytosolic and mitochondrial, can be distinguished by their electrophoretic mobilities, kinetic properties, and subcellular localizations. Most Caucasians have two major isozymes, while approximately 50% of Orientals have only the cytosolic isozyme, missing the mitochondrial isozyme. A remarkably higher frequency of acute alcohol intoxication among Orientals than among Caucasians could be related to the absence of the mitochondrial isozyme. This gene encodes a cytosolic isoform, which has a high affinity for aldehydes. [provided by RefSeq]. |
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Western blot - ALDH1A1 antibody [EP1933Y]; Anti-ALDH1A1 antibody [EP1933Y] at 1/2000 dilution + HepG2 cell lysate at 10 µg.Secondary.HRP labeled Goat anti-rabbit at 1/2000 dilution.Predicted band size : 55 kDa.Observed band size : 55 kDa.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - ALDH1A1 antibody [EP1933Y]; Immunohistochemical analysis of paraffin-embedded human liver using ab52492 at 1/100 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-ALDH1A1 antibody [EP1933Y](ab52492); ab52492 showing positive staining in Normal brain tissue.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-ALDH1A1 antibody [EP1933Y](ab52492); ab52492 showing positive staining in Normal lung tissue.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-ALDH1A1 antibody [EP1933Y](ab52492); ab52492 showing positive staining in Normal kidney tissue.
Immunocytochemistry/ Immunofluorescence - ALDH1A1 antibody [EP1933Y]; ICC/IF image of ab52492 stained MCF7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody overnight at +4°C. The secondary antibody (green) was Alexa Fluor? 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor? 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.