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Anti-AKT3 TRUEMAB Antibody Clone OTI9H8
TrueMAB Antibodies - Made against Authentic Protein Antigens
Also for AKT3 (NM_005465)
|Recombinant fragment expressed in E.coli corresponding to amino acids 2-479 of human AKT3|
|WB, IHC, IF
||WB: 1:500, IHC 1;50, IF (1:100)
|PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.|
|Purified from mouse ascites fluids by affinity chromatography
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY AKT3(RC221051) (RC221051, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-AKT3.
Western blot analysis of extracts (10ug) from 10 Human tissue by using anti-AKT3 monoclonal antibody at 1:200 (1: Testis; 2: Omentum; 3: Uterus; 4: Breast; 5: Brain; 6: Liver; 7: Ovary; 8: Thyroid gland; 9: colon;10: spleen).
Immunohistochemical staining of paraffin-embedded Carcinoma of lung using anti-AKT3 (TA500080) mouse monoclonal antibody (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min).
Anti-AKT3 mouse monoclonal antibody (TA500080) immunofluorescent staining of HeLa cells transiently transfected by pCMV6-ENTRY AKT3(RC221051)
Immunofluorescent staining of HeLa cells using anti-AKT3 mouse monoclonal antibody (TA500080).