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Anti-AKT3 TRUEMAB Antibody Clone OTI9B2
TrueMAB Antibodies - Made against Authentic Protein Antigens
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Also for AKT3 (NM_005465)
|Full length human recombinant protein of human AKT3 (NP_005456) produced in E.coli.|
|WB, IHC, IF, FC
||WB 1:500, IHC 1:50, IF 1:100, FLOW 1:100
|PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.|
|Purified from mouse ascites fluids by affinity chromatography
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY AKT3 (RC221051, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-AKT3.
AKT3 antibody ( 9B2 ) at 1:500 dilution + BALB/C 3T3 cell lysate
Western blot analysis of extracts (10ug) from 2 different cell lines by using anti-AKT3 monoclonal antibody at 1:200 dilution.
Figure from citation: Western Blot of Akt3 protein level by using anti-Akt3 antibody in rat endometrium during pregnancy and pseudopregnancy. View Citation
Immunohistochemical staining of paraffin-embedded Human lung tissue within the normal limits using anti-AKT3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500001)
Anti-AKT3 mouse monoclonal antibody (TA500001) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY AKT3(RC221051).
HEK293T cells transfected with either RC221051 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-AKT3 antibody(TA500001), and then analyzed by flow cytometry.