Anti-AKT3 Antibody, clone 9B2

Also for AKT3 (NM_005465)

OriGene Data

Immunogen	Full length human recombinant protein of human AKT3 (NP_005456) produced in E.coli.
Clone Name	clone 9B2	Isotype	IgG3
Species Reactivity	Human, Mouse	Concentration	0.5~1.0 mg/ml (Lot Dependent)
Guaranteed Application *	WB, IHC, IF, FC	Suggested Dilutions	WB 1:500, IHC 1:50, IF 1:100, FLOW 1:100
Buffer	PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification	Purified from mouse ascites fluids by affinity chromatography (Protein A or G Sepharose)

Reference Data

Target Name	Homo sapiens v-akt murine thymoma viral oncogene homolog 3 (protein kinase B, gamma) (AKT3), transcript variant 1
Alternative Name	MPPH; PKB-GAMMA; PKBG; PRKBG; RAC-gamma; RAC-PK-gamma; STK-2
Database Link	NP_005456
Function	The protein encoded by this gene is a member of the AKT, also called PKB, serine/threonine protein kinase family. AKT kinases are known to be regulators of cell signaling in response to insulin and growth factors. They are involved in a wide variety of biological processes including cell proliferation, differentiation, apoptosis, tumorigenesis, as well as glycogen synthesis and glucose uptake. This kinase has been shown to be stimulated by platelet-derived growth factor (PDGF), insulin, and insulin-like growth factor 1 (IGF1). Alternatively splice transcript variants encoding distinct isoforms have been described.
Related Pathway	Focal Adhesion Hemostasis Jak-STAT signaling pathway MAPK signaling pathway Signaling by GPCR Toll-like receptor signaling pathway

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WB Image

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY AKT3 (RC221051, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-AKT3.

WB Image

AKT3 antibody ( 9B2 ) at 1:500 dilution + BALB/C 3T3 cell lysate

IHC Image

Immunohistochemical staining of paraffin-embedded Human lung tissue using anti-AKT3 mouse monoclonal antibody. (TA500001)

IF Image

Anti-AKT3 mouse monoclonal antibody (TA500001) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY AKT3(RC221051).

FC Image

HEK293T cells transfected with either RC221051 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-AKT3 antibody(TA500001), and then analyzed by flow cytometry.

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OriGene offers bulk sizes of TrueMAB antibodies in both the BSA/glycerol formulation and the carrier-free (BSA/glycerol-free) formulation.

Anti-AKT3 TrueMAB Antibody clone 9B2

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

OriGene Data

Reference Data