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Home Antibody All anti-AKT1S1 antibodies

Anti-AKT1S1 Antibody EPR6262

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA310181
  • Rabbit Monoclonal antibody against PRAS40 (AKT1S1)
  • FREE positive control: HEK293T cell transient overexpression lysate (LC410168) , 20ug
100ul $325 3-7 Days
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WB(1)
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Also for AKT1S1 (NM_032375)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenA synthetic peptide corresponding to residues near the C-terminus of human PRAS40 was used as an immunogen.
Clone NameEPR6262 IsotypeIgG
Species ReactivityHuman, Mouse, Rat Concentration0.5~1.0 mg/ml (Lot Dependent)
Guaranteed Application *WB Suggested DilutionsWB: 1:1000 - 1:10000; IP: 1:10 - 1:100
BufferpH: 7.40
Preservative: 0.01% Sodium azide
Constituents: 50% Glycerol, 0.05% BSA
Purification Tissue culture supernatant
Note Is unsuitable for Flow Cyt,ICC or IHC-P.

Reference Data

Target NameHomo sapiens AKT1 substrate 1 (proline-rich) (AKT1S1), transcript variant 1
Alternative NameLobe; PRAS40
Database LinkNP_115751
FunctionPRAS40 is a novel substrate of Akt, the phosphorylation of which leads to the binding of this protein to 14-3-3 (1). It is a subunit of mTORC1, which regulates cell growth and survival in response to nutrient and hormonal signals. mTORC1 is activated in response to growth factors or amino-acids. Growth factor-stimulated mTORC1 activation involves a AKT1-mediated phosphorylation of TSC1-TSC2, which leads to the activation of the RHEB GTPase that potently activates the protein kinase activity of mTORC1 (2).
Related Pathway

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WB Image
Western blot - Anti-PRAS40 antibody [EPR6262]; All lanes : Anti-PRAS40 antibody [EPR6262] at 1/1000 dilution.Lane 1 : NIH/3T3 cell lysate.Lane 2 : L6 cell lysate.Lane 3 : HeLa cell lysate.Lane 4 : 293T cell lysate.Lysates/proteins at 10 µg per lane.Secondary.HRP labelled goat anti-rabbit at 1/2000 dilution.Predicted band size : 27 kDa.

 

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