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Anti-AKT1 PHOSPHO Antibody EPR4157
Also for AKT1 (NM_005163)
|A phospho specific peptide corresponding to residues surrounding threonine 450 of human Akt1 was used as an immunogen. This antibody only detects Akt1 phosphorylated at threonine 450.|
|Human, Mouse, Rat
||0.5~1.0 mg/ml (Lot Dependent)
||WB: 1:1000 - 1:10000; IP: 1:10 - 1:100; IHC-P: 1:50 - 1:100
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant (Protein A or G Sepharose)
|Is unsuitable for Flow Cyt or ICC.
|Homo sapiens v-akt murine thymoma viral oncogene homolog 1 (AKT1), transcript variant 1|
|AKT; CWS6; PKB; PKB-ALPHA; PRKBA; RAC; RAC-ALPHA|
|Akt1/PKBa is a Serine/Threonine protein kinase whose kinase activity plays a key role in various cellular functions, including apoptosis, glycogen synthesis, and cell growth. Akt1/PKBa is activated by various growth and survival factors (1). Akt1/PKBa inhibits apoptosis by phosphorylating and inactivating several targets, including caspase-9 (2). Akt1/PKBa regulates glycogen synthesis by phosphorylating and inactivating glycogen synthase kinase-3a and b (3,4).|
Delta-Notch Signaling Pathway
EGFR1 Signaling Pathway
Jak-STAT signaling pathway
MAPK signaling pathway
Signaling by GPCR
Toll-like receptor signaling pathway
Wnt Signaling Pathway
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Western blot - AKT1 (phospho T450) antibody [EPR4157]; All lanes : Anti-AKT1 (phospho T450) antibody [EPR4157] at 1/1000 dilution.Lane 1 : NIH3T3 cell lysate, untreated .Lane 2 : NIH3T3 cell lysate, treated with Lambda Phosphatase.Lysates/proteins at 10 µg per lane.Predicted band size : 56 kDa.Observed band size : 60 kDa .
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - AKT1 (phospho T450) antibody [EPR4157]; ab108266 at 1/50 dilution staining AKT1 in Human breast carcinom by Immunohistochemistry, Paraffin-embedded tissue.