Anti-AKT1 Antibody,

Also for AKT1 (NM_001014431)

OriGene Data

Immunogen	Akt1 antibody was raised against a 16 amino acid peptide from near the amino-terminus of human Akt1.
Clone Name		Isotype	IgG
Species Reactivity	Human, Mouse, Rat	Concentration	1ug/ul
Guaranteed Application *	WB, IHC	Suggested Dilutions	WB: 1 ug/ml
Buffer	PBS containing 0.02% sodium azide.
Purification	Affinity chromatography purified via peptide column (Protein A or G Sepharose)

Reference Data

Target Name	Homo sapiens v-akt murine thymoma viral oncogene homolog 1 (AKT1), transcript variant 3
Alternative Name	AKT; PKB; PKB-ALPHA; PRKBA; RAC; RAC-ALPHA
Database Link	NP_001014431
Function	Akt1, initially identified as the cellular homolog to the retro-viral oncogene v-Akt (1), is part of the phosphatidyl 3’-kinase (PI3K)-Akt signaling pathway that is activated by diverse cellular stimuli and regulates critical cellular functions such as cell growth, proliferation, and survival (2). Following phosphorylation of the second messenger PIP2 by PI3K, Akt1 translocates to the cell membrane where it is activated by phosphoinositide-dependent kinase (PDK) 1 and PDK2 (3). The active Akt1 is then able to phosphorylate and activate its substrates, including those that are important for cell proliferation and survival such as TOR and the Bcl-2 homolog Bad (4,5). Negative regulation of the PI3K-Akt signaling pathway is mainly accomplished by the lipid phosphatase activity of PTEN which catalyzes the conversion of PIP3 to PIP2, thereby preventing the activation of Akt1. Inactivation of this gene often results in excessive Akt1 activity, often leading to the formation of malignant tumors (reviewed in 6).
Related Pathway	Apoptosis Delta-Notch Signaling Pathway EGFR1 Signaling Pathway Focal Adhesion Hemostasis Jak-STAT signaling pathway MAPK signaling pathway Signaling by GPCR Toll-like receptor signaling pathway Wnt Signaling Pathway

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WB Image

Western blot analysis of Akt1 in human liver cell lysate with Akt1 antibody at (A) 1 and (B) 2 ug/ml.

IHC Image

Immunohistochemical staining of A431 cells using Beclin-1 antibody at 1 ug/ml.

Conjugation Services
OriGene provides conjugation services of TrueMAB antibodies with the following molecules:

HRP
Biotin
PE
FITC
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DyLight® 594
DyLight® 633

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Bulk Order
OriGene offers bulk sizes of TrueMAB antibodies in both the BSA/glycerol formulation and the carrier-free (BSA/glycerol-free) formulation.

Anti-AKT1 Antibody

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

OriGene Data

Reference Data