Home Antibody All anti-AKT1 antibodies
Anti-AKT1 Antibody Y89
Also for AKT1 (NM_001014432)
|A synthetic peptide corresponding to residues in C-terminus of human Akt1 was used as immunogen. Predicted to cross-react with mouse, rat and bovine, based on sequence homology.|
||Lot dependent; please refer to CoA along with shipment
|WB, IHC, IF, FC
||ICC/IF: 1:100 - 1:250; WB: 1:5000 - 1:10000; IHC-P: Use at an assay dependent dilution; FC: 1:20; IP: 1:100
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Protein A purified
* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.
Western blot - AKT1 antibody [Y89]; Anti-AKT1 antibody [Y89] at 1/10000 dilution + MCF-7 cell lysate.Predicted band size : 56 kDa.Observed band size : 59 kDa .
Immunohistochemistry (Paraffin-embedded sections) - AKT1 antibody [Y89]; Immunohistochemical analysis of paraffin-embedded prostate carcinoma using TA303387 at 1/100 dilution.
Immunocytochemistry/ Immunofluorescence - Anti-AKT1 antibody [Y89]; TA303387 staining AKT1 in SK-N-SH cells treated with alsterpaullone , by ICC/IF. Decrease of AKT1 expression correlates with increased concentration of alsterpaullone, as described in literature.The cells were incubated at 37Â°C for 6h in media containing different concentrations of (alsterpaullone) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with TA303387 (1/200 dilution was performed overnight at 4Â°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 anti-rabbit polyclonal antibody at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.
Flow Cytometry-AKT1 antibody [Y89](TA303387); Overlay histogram showing HeLa cells stained with TA303387 (red line). The cells were fixed with methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was DyLight 488 goat anti-rabbit IgG (H+L) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit monoclonal IgG (1ug/1x10^6 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a slightly decreased signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.