OriGene Technologies, Inc.
Search:    
Left ProductsProducts divider ServicesServices divider technologyTechnology divider researchResearch divider TechsupportTechSupport divider AboutAbout Right
 
Home Antibody All anti-AKT1 antibodies

Anti-AKT1 Antibody Y89

div

Specifications Citations (0) Related Products Product Documents
SKU Description Amount Price Availability*  
TA303387 Rabbit monoclonal antibody against Akt1/PKBa (C-term)(Y89) 100ul $325 In Stock
LC423057 AKT1 HEK293T cell transient overexpression lysate (as WB positive control) 20ug $50 In Stock
Add to Shopping Cart
spacer
WB(1)
IHC(1)
IF(1)
FC(1)
spacer
Also for AKT1 (NM_001014432)
cDNA Clone shRNA/siRNA CRISPR KO Kit Protein Antibody

OriGene Data

ImmunogenA synthetic peptide corresponding to residues in C-terminus of human Akt1 was used as immunogen. Predicted to cross-react with mouse, rat and bovine, based on sequence homology.
Clone NameY89 IsotypeIgG
Species ReactivityMouse, Human ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, IHC, IF, FC Suggested DilutionsICC/IF: 1:100 - 1:250; WB: 1:5000 - 1:10000; IHC-P: Use at an assay dependent dilution; FC: 1:20; IP: 1:100
Predicted MW Explanation 56 kDa
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification Protein A purified

Reference Data

Target NameHomo sapiens v-akt murine thymoma viral oncogene homolog 1 (AKT1), transcript variant 2
Alternative NameAKT; CWS6; PKB; PKB-ALPHA; PRKBA; RAC; RAC-ALPHA
Database LinkNP_001014432
Entrez Gene 207 Human
Entrez Gene 11651 Mouse
FunctionThe serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq].
Related PathwayProtein KinaseES Cell Differentiation/IPSDruggable Genome MAPK signaling pathwayErbB signaling pathwayChemokine signaling pathwaymTOR signaling pathwayApoptosisVEGF signaling pathwayMore Pathways >>

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

Western blot - AKT1 antibody [Y89]; Anti-AKT1 antibody [Y89] at 1/10000 dilution + MCF-7 cell lysate.Predicted band size : 56 kDa.Observed band size : 59 kDa .
spacer
Immunohistochemistry (Paraffin-embedded sections) - AKT1 antibody [Y89]; Immunohistochemical analysis of paraffin-embedded prostate carcinoma using TA303387 at 1/100 dilution.
spacer
ICC/IF - Anti-AKT1 antibody; TA303387 staining AKT1 in SK-N-SH cells treated with alsterpaullone , by ICC/IF.The cells were incubated at 37°C for 6h in media containing different concentrations of (alsterpaullone) in DMSO. Staining of the treated cells with TA303387 (1/200 dilution was performed overnight at 4°C. A DyLight 488 anti-rabbit polyclonal antibody at 1:250 was used as the secondary antibody. Nuclei were counterstained with DAPI.
spacer
Flow Cytometry-AKT1 antibody(TA303387); Overlay histogram showing HeLa cells stained with TA303387 (red line). The secondary antibody used was DyLight 488 goat anti-rabbit IgG (H+L) at 1:500. Isotype control antibody (black line) was rabbit monoclonal IgG (1ug/1x10^6 cells) used under the same conditions. This antibody gave a slightly decreased signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.
spacer

 

spacer
Inc 5000 Healthcare Company
All Products by: Title | Price | Category | Popularity | Best Sellers Topselling Products by: Title | Price | Category | Popularity | Favorites
Popular Categories: Popularity | Our Choices | All-Round Favorites | Title Topselling Categories: Popularity | Our Choices | All-Round Favorites | Title

Notice to Non-US Customers

ExclaimationWeb price and delivery time are for direct sales only.

Non-US customers are encouraged to contact our dedicated distributor network for quotes and streamlined ordering/delivery support.

Acknowledge