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Home Antibody All anti-AK1 antibodies

Anti-AK1 TRUEMAB Antibody Clone OTI18C7

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

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Specifications Citations (0) Related Products Product Documents
SKU Description Amount Price Availability*  
TA500323 AK1 (Adenylate Kinase 1) mouse monoclonal antibody, clone OTI18C7 (formerly 18C7) 100ul $325 In Stock
LC400171 AK1 HEK293T cell transient overexpression lysate (as WB positive control) 20ug $50 In Stock
CF500323 Carrier-free (BSA/glycerol-free) AK1 mouse monoclonal antibody, clone OTI18C7 (formerly 18C7) 100ug $450 3-4 weeks
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WB(2)
IF(1)
FC(1)

OriGene Data

ImmunogenFull length human recombinant protein of human AK1 (NP_000467) produced in HEK293T cell.
Clone NameClone OTI18C7 IsotypeIgG1
Species ReactivityHuman Concentration0.31 mg/ml
Guaranteed Application *WB, IF, FC Suggested DilutionsWB 1:1000~2000, IF 1:100, FLOW 1:100
Predicted MW Explanation 21.6 kDa
BufferPBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from mouse ascites fluids by affinity chromatography

Reference Data

Target NameHomo sapiens adenylate kinase 1 (AK1)
Alternative NameHTL-S-58j
Database LinkNP_000467
Entrez Gene 203 Human
FunctionAdenylate kinase is an enzyme involved in regulating the adenine nucleotide composition within a cell by catalyzing the reversible transfer of phosphate group among adinine nucleotides. Three isozymes of adenylate kinase have been identified in vertebrates, adenylate isozyme 1 (AK1), 2 (AK2) and 3 (AK3). AK1 is found in the cytosol of skeletal muscle, brain and erythrocytes, whereas AK2 and AK3 are found in the mitochondria of other tissues including liver and heart. AK1 was identified because of its association with a rare genetic disorder causing nonspherocytic hemolytic anemia where a mutation in the AK1 gene was found to reduce the catalytic activity of the enzyme.
Related PathwayDruggable Genome Purine metabolismMetabolic pathways

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY AK1 (RC215130, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-AK1.
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Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-AK1 monoclonal antibody.
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Anti-AK1 mouse monoclonal antibody (TA500323) immunofluorescent staining of Hela cells transiently transfected by pCMV6-ENTRY AK1(RC215130).
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HEK293T cells transfected with either RC215130 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-AK1 antibody(TA500323), and then analyzed by flow cytometry.
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