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Anti-AK1 TRUEMAB Antibody clone 19G4
TrueMAB Antibodies - Made against Authentic Protein Antigens
|Full length human recombinant protein of human AK1 (NP_000467) produced in HEK293T cell.|
||WB 1:500~1000, FLOW 1:100
|PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.|
|Purified from mouse ascites fluids by affinity chromatography (Protein A or G Sepharose)
|Homo sapiens adenylate kinase 1 (AK1)|
|ATP-AMP transphosphorylase; OTTHUMP00000022217; OTTHUMP00000022218; myokinase; adenylate kinase 1|
|Adenylate kinase is an enzyme involved in regulating the adenine nucleotide composition within a cell by catalyzing the reversible transfer of phosphate group among adinine nucleotides. Three isozymes of adenylate kinase have been identified in vertebrates, adenylate isozyme 1 (AK1), 2 (AK2) and 3 (AK3). AK1 is found in the cytosol of skeletal muscle, brain and erythrocytes, whereas AK2 and AK3 are found in the mitochondria of other tissues including liver and heart. AK1 was identified because of its association with a rare genetic disorder causing nonspherocytic hemolytic anemia where a mutation in the AK1 gene was found to reduce the catalytic activity of the enzyme.|
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY AK1 (RC215130, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-AK1.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-AK1 monoclonal antibody.
HEK293T cells transfected with either RC215130 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-AK1 antibody(TA500320), and then analyzed by flow cytometry.