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Also for ADD1 (NM_001119)
|The antiserum was produced against synthesized non-phosphopeptide derived from human ADD1 around the phosphorylation site of serine 726 (T-P-SP-F-L).|
|Human, Mouse, Rat
|WB, IHC, IF
||WB: 1:500~1000, IHC: 1:50~100, IP: Various DilutionIF: 1:100~1:500, ELISA: 1:40000
|Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.|
|The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. (Protein A or G Sepharose)
|ADD1 (Ab-726) antibody detects endogenous levels of total ADD1 protein.
|Homo sapiens adducin 1 (alpha) (ADD1), transcript variant 1|
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Western blot analysis of extracts from Hela cells treated with Forskolin (40nM, 30min), using ADD1 (Ab-726) antibody (#B0002, Line 1 and 2).The lane on the right is treated with the synthesized peptide.
Immunohistochemical analysis of paraffin-embedded human brain tissue using ADD1 (Ab-726) antibody (#B0002).The picture on the right is treated with the synthesized peptide.
Immunofluorescence analysis of HeLa cells, using ADD1 (Ab-726) antibody (#B0002).The picture on the right is treated with the synthesized peptide.