Anti-ACY1 Antibody, clone 1A12

Also for ACY1 (NM_000666)

OriGene Data

Immunogen	Full length human recombinant protein of human ACY1(NP_000657) produced in HEK293 cell.
Clone Name	clone 1A12	Isotype	IgG1
Species Reactivity	Human , Dog , Rat , Mouse	Concentration	0.5~1.0 mg/ml (Lot Dependent)
Guaranteed Application *	WB, IHC, FC	Suggested Dilutions	WB 1:500~2000, IHC 1:150, FLOW 1:100,
Buffer	PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification	Purified from mouse ascites fluids by affinity chromatography (Protein A or G Sepharose)

Reference Data

Target Name	Homo sapiens aminoacylase 1 (ACY1), transcript variant 1
Alternative Name	ACY-1; ACY1D
Database Link	NP_000657
Function	This gene encodes a cytosolic, homodimeric, zinc-binding enzyme that catalyzes the hydrolysis of acylated L-amino acids to L-amino acids and an acyl group, and has been postulated to function in the catabolism and salvage of acylated amino acids. This gene is located on chromosome 3p21.1, a region reduced to homozygosity in small-cell lung cancer (SCLC), and its expression has been reported to be reduced or undetectable in SCLC cell lines and tumors. The amino acid sequence of human aminoacylase-1 is highly homologous to the porcine counterpart, and this enzyme is the first member of a new family of zinc-binding enzymes. Mutations in this gene cause aminoacylase-1 deficiency, a metabolic disorder characterized by central nervous system defects and increased urinary excretion of N-acetylated amino acids. Alternative splicing of this gene results in multiple transcript variants. Read-through transcription also exists between this gene and the upstream ABHD14A (abhydrolase domain containing 14A) gene, as represented in GeneID:100526760. A related pseudogene has been identified on chromosome 18. [provided by RefSeq].
Related Pathway

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WB Image

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ACY1 (RC201284, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ACY1.

WB Image

Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-ACY1 monoclonal antibody.

IHC Image

Immunohistochemical staining of paraffin-embedded Human Kidney tissue using anti-ACY1 mouse monoclonal antibody. (TA503226)

IHC Image

Immunohistochemical staining of paraffin-embedded Human liver tissue using anti-ACY1 mouse monoclonal antibody. (TA503226)

IHC Image

Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-ACY1 mouse monoclonal antibody. (TA503226)

FC Image

HEK293T cells transfected with either RC201284 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-ACY1 antibody(TA503226), and then analyzed by flow cytometry.

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Anti-ACY1 TrueMAB Antibody clone 1A12

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

OriGene Data

Reference Data