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Anti-ACY1 TRUEMAB Antibody Clone 2F1

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA503213
  • ACY1 (Aminoacylase 1) mouse monoclonal antibody, Clone 2F1
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC424578) , 20ug Explanation
100ul 325 In Stock
CF503213
  • Carrier-free (BSA/glycerol-free) ACY1 mouse monoclonal antibody,clone 2F1
100ug $450 3-4 weeks
This antibody qualifies for Season of Giving promotion: Earn $50 Amazon gift card (use promo code SPQ12081) or donation to Cancer.org (promo code SPQ12082) with $600 purchase of UltraMAB or TrueMAB. Click for details
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WB(2)
IHC(9)
FC(1)
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Also for ACY1 (NM_000666)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenFull length human recombinant protein of human ACY1(NP_000657) produced in HEK293T cell.
Clone NameClone 2F1 IsotypeIgG1
Species ReactivityHuman , Dog , Monkey Concentration0.54 mg/ml
Guaranteed Application *WB, IHC, FC Suggested DilutionsWB 1:500~2000, IHC 1:150, FLOW 1:100,
Predicted MW Explanation 45.7 kDa
BufferPBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from mouse ascites fluids by affinity chromatography

Reference Data

Target NameHomo sapiens aminoacylase 1 (ACY1), transcript variant 1
Alternative NameACY-1; ACY1D; HEL-S-5
Database LinkNP_000657
Entrez Gene 95 Human
Entrez Gene 476602 Dog
FunctionThis gene encodes a cytosolic, homodimeric, zinc-binding enzyme that catalyzes the hydrolysis of acylated L-amino acids to L-amino acids and an acyl group, and has been postulated to function in the catabolism and salvage of acylated amino acids. This gene is located on chromosome 3p21.1, a region reduced to homozygosity in small-cell lung cancer (SCLC), and its expression has been reported to be reduced or undetectable in SCLC cell lines and tumors. The amino acid sequence of human aminoacylase-1 is highly homologous to the porcine counterpart, and this enzyme is the first member of a new family of zinc-binding enzymes. Mutations in this gene cause aminoacylase-1 deficiency, a metabolic disorder characterized by central nervous system defects and increased urinary excretion of N-acetylated amino acids. Alternative splicing of this gene results in multiple transcript variants. Read-through transcription also exists between this gene and the upstream ABHD14A (abhydrolase domain containing 14A) gene, as represented in GeneID:100526760. A related pseudogene has been identified on chromosome 18. [provided by RefSeq].
Related PathwayProtease Arginine and proline metabolismMetabolic pathways

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ACY1 (RC201284, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ACY1.
WB Image
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-ACY1 monoclonal antibody.
IHC Image
Immunohistochemical staining of paraffin-embedded Human breast tissue using anti-ACY1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503213)
IHC Image
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-ACY1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503213)
IHC Image
Immunohistochemical staining of paraffin-embedded Human Kidney tissue using anti-ACY1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503213)
IHC Image
Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-ACY1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503213)
IHC Image
Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-ACY1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503213)
IHC Image
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-ACY1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503213)
IHC Image
Immunohistochemical staining of paraffin-embedded Human prostate tissue using anti-ACY1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503213)
IHC Image
Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-ACY1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503213)
IHC Image
Immunohistochemical staining of paraffin-embedded Human tonsil using anti-ACY1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503213)
FC Image
HEK293T cells transfected with either RC201284 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-ACY1 antibody(TA503213), and then analyzed by flow cytometry.

 

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