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Home Antibody All anti-ACY1 antibodies

Anti-ACY1 TRUEMAB Antibody Clone OTI1D4

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

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Specifications Citations Customer Reviews Product Documents
SKU Description Amount Price Availability*  
TA503189 ACY1 (Aminoacylase 1) mouse monoclonal antibody, clone OTI1D4 (formerly 1D4) 100ul $325 In Stock
LC424578 ACY1 HEK293T cell transient overexpression lysate (as WB positive control) 20ug $50 In Stock
CF503189 Carrier-free (BSA/glycerol-free) ACY1 mouse monoclonal antibody, clone OTI1D4 (formerly 1D4) 100ug $450 4 Weeks
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WB(2)
IHC(5)
FC(1)
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OriGene Data

ImmunogenFull length human recombinant protein of human ACY1(NP_000657) produced in HEK293 cell.
Clone NameClone OTI1D4 IsotypeIgG1
Species ReactivityHuman, Mouse Concentration0.3 mg/ml
Guaranteed Application *WB, IHC, FC Suggested DilutionsWB 1:500~2000, IHC 1:150, FLOW 1:100,
Predicted MW Explanation 45.7 kDa
BufferPBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from mouse ascites fluids by affinity chromatography

Reference Data

Target NameHomo sapiens aminoacylase 1 (ACY1), transcript variant 1
Alternative NameACY-1; ACY1D; HEL-S-5
Database LinkNP_000657
Entrez Gene 95 Human
Entrez Gene 109652 Mouse
FunctionThis gene encodes a cytosolic, homodimeric, zinc-binding enzyme that catalyzes the hydrolysis of acylated L-amino acids to L-amino acids and an acyl group, and has been postulated to function in the catabolism and salvage of acylated amino acids. This gene is located on chromosome 3p21.1, a region reduced to homozygosity in small-cell lung cancer (SCLC), and its expression has been reported to be reduced or undetectable in SCLC cell lines and tumors. The amino acid sequence of human aminoacylase-1 is highly homologous to the porcine counterpart, and this enzyme is the first member of a new family of zinc-binding enzymes. Mutations in this gene cause aminoacylase-1 deficiency, a metabolic disorder characterized by central nervous system defects and increased urinary excretion of N-acetylated amino acids. Alternative splicing of this gene results in multiple transcript variants. Read-through transcription also exists between this gene and the upstream ABHD14A (abhydrolase domain containing 14A) gene, as represented in GeneID:100526760. A related pseudogene has been identified on chromosome 18. [provided by RefSeq].
Related PathwayProtease Arginine and proline metabolismMetabolic pathways

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.


WB Image
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ACY1 (RC201284, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ACY1.
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WB Image
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-ACY1 monoclonal antibody.
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IHC Image
Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-ACY1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503189)
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IHC Image
Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-ACY1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503189)
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IHC Image
Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-ACY1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503189)
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IHC Image
Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-ACY1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503189)
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IHC Image
Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-ACY1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503189)
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FC Image
HEK293T cells transfected with either RC201284 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-ACY1 antibody(TA503189), and then analyzed by flow cytometry.
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