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Home Antibody All anti-ACTL6A antibodies

Anti-ACTL6A Antibody EPR7443

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Specifications Citations (0) Related Products Product Documents
SKU Description Amount Price Availability*  
TA310170
  • Rabbit Monoclonal antibody against BAF53 (ACTL6A)
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC418091) , 20ug Explanation
100ul $325 3-7 Days
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WB(1)
IHC(2)
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Also for ACTL6A (NM_004301)
cDNA Clone shRNA/siRNA CRISPR KO Kit Protein Antibody

OriGene Data

ImmunogenA synthetic peptide corresponding to residues in human BAF53 was used as an immunogen.
Clone NameEPR7443 IsotypeIgG
Species ReactivityMouse, Rat, Human ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, IHC Suggested DilutionsWB: 1:1000 - 1:10000; IP: 1:10 - 1:100; IHC-P: 1:50 - 1:100; ICC/IF: 1:100 - 1:250
Predicted MW Explanation 47 kDa
BufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 49% PBS, 50% Glycerol, 0.05% BSA
Purification Tissue culture supernatant
Note Is unsuitable for Flow Cyt.

Reference Data

Target NameHomo sapiens actin-like 6A (ACTL6A), transcript variant 1
Alternative NameACTL6; Arp4; ARPN-BETA; BAF53A; INO80K
Database LinkNP_004292
Entrez Gene 86 Human
Entrez Gene 56456 Mouse
Entrez Gene 361925 Rat
FunctionBAF53 is a family member of actin-related proteins (ARPs) that share significant amino acid sequence identity to conventional actins. Both actins and ARPs have an actin fold, which is an ATP-binding cleft, as a common feature. The ARPs are involved in diverse cellular processes, including vesicular transport, spindle orientation, nuclear migration and chromatin remodeling. BAF53 is a 53 kDa subunit protein of the BAF (BRG1/brm-associated factor) complex in mammals, which is functionally related to SWI/SNF complex in S. cerevisiae and Drosophila; the latter is thought to facilitate activation of specific proteins by antagonizing chromatin-mediated repression. Together with beta-actin, it is required for maximal ATPase activity of BRG1 and the association of the BAF complex with chromatin/matrix (1).
Related PathwayTranscription FactorsDruggable Genome

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot - Anti-ACTL6A antibody [EPR7443]; All lanes : Anti-ACTL6A antibody [EPR7443] at 1/1000 dilution.Lane 1 : Ramos cell lysate.Lane 2 : K562 cell lysate.Lane 3 : HeLa cell lysate.Lane 4 : Jurkat cell lysate.Lysates/proteins at 10 ug per lane.Secondary.HRP labelled Goat anti-Rabbit IgG at 1/2000 dilution.Predicted band size : 47 kDa.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACTL6A antibody [EPR7443]; Immunohistochemical analysis of paraffin embedded Human breast carcinoma tissue labelling ACTL6A with TA310170 at 1/50 dilution.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACTL6A antibody [EPR7443]; Immunohistochemical analysis of paraffin embedded Human colon tissue labelling ACTL6A with TA310170 at 1/50 dilution.

 

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