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Home Antibody All anti-ACTB antibodies

Anti-ACTB Antibody EP1123Y

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA303377
  • Rabbit monoclonal antibody against B-Actin (Cytoplasmic)(EP1123Y)
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC420311) , 20ug Explanation
100ul 325 In Stock
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WB(1)
IHC(2)
FC(1)
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Also for ACTB (NM_001101)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenA synthetic peptide corresponding to residues near the C-terminus of human Beta Actin (Cytoplasmic Actin) was used as an immunogen.
Clone NameEP1123Y IsotypeIgG
Species ReactivityMouse, Rat, Human ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, IHC, FC Suggested DilutionsFC: 1:1000; ICC/IF: 1:50; WB: 1:500; IHC-P: Use at an assay dependent concentration
Predicted MW Explanation 42 kDa
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification Tissue culture supernatant
Note Is unsuitable for IP.

Reference Data

Target NameHomo sapiens actin, beta (ACTB)
Alternative NameBRWS1; PS1TP5BP1
Database LinkNP_001092
Entrez Gene 60 Human
Entrez Gene 11461 Mouse
Entrez Gene 81822 Rat
FunctionThis gene encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. This actin is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins. [provided by RefSeq].
Related PathwayES Cell Differentiation/IPS Focal adhesionAdherens junctionTight junctionLeukocyte transendothelial migrationRegulation of actin cytoskeletonVibrio cholerae infectionMore Pathways >>

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot - beta Actin antibody [EP1123Y]; All lanes : Anti-beta Actin antibody [EP1123Y] at 1/500 dilution.Lane 1 : T47D cell lysate.Lane 2 : HeLa cell lysate.Lysates/proteins at 10 ug per lane.Secondary.Goat anti-rabbit HRP antibody at 1/2000 dilution.Predicted band size : 42 kDa.Observed band size : 42 kDa.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Actin antibody [EP1123Y]; TA303377 stainingbeta Actinin Mouse embryo tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 1% FBS/BSA for1 hourat room temperature; antigen retrieval was by heat mediation incitrate buffer, pH6. Samples were incubated with primary antibody (1/100 in1% FBS/BSA) for 16 hours at 4°C. An undilutedHRP-conjugatedGoat anti-rabbit IgG polyclonalwas used as the secondary antibody.
IHC Image
Immunohistochemistry (Paraffin-embedded sections) - beta Actin antibody [EP1123Y]; Ab52614 at 1/50 dilution staining human muscle; paraffin embedded.
FC Image
Flow Cytometry - Anti-beta Actin antibody [EP1123Y]; Overlay histogram showing HeLa cells stained with TA303377 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was Alexa Fluor 488 goat anti-rabbit IgG (H+L) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1ug/1x10^6 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

 

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