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Home Antibody All anti-ACTB antibodies

Anti-ACTB Antibody EP1123Y

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SKU Description Amount Price Availability*  
TA303377 Rabbit monoclonal antibody against B-Actin (Cytoplasmic)(EP1123Y) 100ul $325 In Stock
LC420311 ACTB HEK293T cell transient overexpression lysate (as WB positive control) 20ug $50 In Stock
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WB(1)
IHC(2)
FC(1)

OriGene Data

ImmunogenA synthetic peptide corresponding to residues near the C-terminus of human Beta Actin (Cytoplasmic Actin) was used as an immunogen.
Clone NameEP1123Y IsotypeIgG
Species ReactivityMouse, Rat, Human ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, IHC, FC Suggested DilutionsFC: 1:1000; ICC/IF: 1:50; WB: 1:500; IHC-P: Use at an assay dependent concentration
Predicted MW Explanation 42 kDa
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification Tissue culture supernatant
Note Is unsuitable for IP.

Reference Data

Target NameHomo sapiens actin, beta (ACTB)
Alternative NameBRWS1; PS1TP5BP1
Database LinkNP_001092
Entrez Gene 60 Human
Entrez Gene 11461 Mouse
Entrez Gene 81822 Rat
FunctionThis gene encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. This actin is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins. [provided by RefSeq].
Related PathwayES Cell Differentiation/IPS Focal adhesionAdherens junctionTight junctionLeukocyte transendothelial migrationRegulation of actin cytoskeletonVibrio cholerae infectionMore Pathways >>

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

Western blot - beta Actin antibody [EP1123Y]; All lanes : Anti-beta Actin antibody [EP1123Y] at 1/500 dilution.Lane 1 : T47D cell lysate.Lane 2 : HeLa cell lysate.Lysates/proteins at 10 ug per lane.Secondary.Goat anti-rabbit HRP antibody at 1/2000 dilution.Predicted band size : 42 kDa.Observed band size : 42 kDa.
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IHC - Anti-beta Actin antibody; TA303377 stainingbeta Actinin Mouse embryo tissue sections by IHC (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 1% FBS/BSA for1 hourat RT; antigen retrieval was by heat mediation incitrate buffer, pH6. Samples were incubated with primary antibody (1/100 in1% FBS/BSA) for 16 hours at 4°C. An undilutedHRP-conjugatedGoat anti-rabbit IgG polyclonalwas used as the secondary antibody.
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Immunohistochemistry (Paraffin-embedded sections) - beta Actin antibody [EP1123Y]; Ab52614 at 1/50 dilution staining human muscle; paraffin embedded.
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Flow Cytometry - Anti-beta Actin antibody; Overlay histogram showing HeLa cells stained with TA303377 (red line). The secondary antibody used was Alexa Fluor 488 goat anti-rabbit IgG (H+L) at 1:2000. Isotype control antibody (black line) was rabbit IgG (monoclonal) used under the same conditions. Unlabelled sample (blue line) was also used as a control. This antibody gave a positive signal in HeLa cells under the same conditions.
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