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Anti-ACO1 Antibody EPR7225
Also for ACO1 (NM_002197)
|A synthetic peptide corresponding to residues in human ACO1 was used as an immunogen.|
|Human, Mouse, Rat
||0.5~1.0 mg/ml (Lot Dependent)
|WB, ASSAY, IF
||WB: 1:1000 - 1:10000; IP: 1:10 - 1:100; ICC/IF: 1:100 - 1:250
Preservative: 0.01% Sodium azide
Constituents: 49% PBS, 50% Glycerol, 0.05% BSA
|Tissue culture supernatant (Protein A or G Sepharose)
|Is unsuitable for Flow Cyt or IHC-P.
|Homo sapiens aconitase 1, soluble (ACO1), transcript variant 2|
|ACONS; IREB1; IREBP; IREBP1; IRP1|
|Aconitase 1, also known as iron regulatory element binding protein 1 (IREB1) or ACO1, is a cytosolic protein that binds to iron-responsive elements (IREs). IREs are stem-loop structures found in the 5' UTR of ferritin mRNA, and in the 3' UTR of transferrin receptor mRNA. The iron-induced binding to the IRE results in repression of translation of ferritin mRNA, and inhibition of degradation of the otherwise rapidly degrading transferrin receptor mRNA. Thus, IREB1 plays a central role in cellular iron homeostasis. ACO1 was also shown to have aconitase activity, and hence grouped with the aconitase family of enzymes (1).|
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Western blot - Anti-Aconitase 1 antibody [EPR7225]; All lanes : Anti-Aconitase 1 antibody [EPR7225] at 1/1000 dilution.Lane 1 : Human thyroid lysate.Lane 2 : HeLa cell lysate.Lane 3 : Human fetal kidney lysate.Lane 4 : HepG2 cell lysate.Lane 5 : T.T. cell lysate.Lysates/proteins at 10 µg per lane.Secondary.Goat anti-rabbit HRP at 1/2000 dilution.Predicted band size : 98 kDa.
Other-Anti-Aconitase 1 antibody [EPR7225](ab126595); Equilibrium disassociation constant (KD)..
Immunocytochemistry/ Immunofluorescence - Anti-Aconitase 1 antibody [EPR7225]; ab126595 at 1/100 dilution staining Aconitase 1 in HeLa cells by immunofluorescence.