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Home Antibody All anti-ACLY antibodies

Anti-ACLY Antibody EP737Y

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA300817
  • Rabbit Monoclonal Antibody against ACLY (Clone EP737Y)
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC420122) , 20ug
100ul 325 3-7 Days
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WB(1)
IF(1)
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Also for ACLY (NM_001096)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenA synthetic phospho-peptide corresponding to residues surrounding Thr447 and Ser451 of human ATP-citrate (pro-S) lyase was used as immunogen. The antibody only detects ATP-citrate (pro-S) lyase phosphorylated on Threonine 447 and Serine 451.
Clone NameEP737Y IsotypeIgG
Species ReactivityHuman, Mouse, Rat ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, IF Suggested DilutionsWB: 1:1000; ICC/IF: 1:100 - 1:250; IP: 1:10 - 1:100
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification Tissue culture supernatant
Note Is unsuitable for Flow Cyt or IHC-P.

Reference Data

Target NameHomo sapiens ATP citrate lyase (ACLY), transcript variant 1
Alternative NameACL; ATPCL; CLATP
Database LinkNP_001087
FunctionATP citrate lyase (ACLY), also known as ATP citrate synthase, is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA in many tissues. The enzyme is a tetramer of four identical subunits. It catalyzes the formation of acetyl-CoA and oxaloacetate from citrate and CoA with a concomitant hydrolysis of ATP to ADP and phosphate. One of these products, acetyl-CoA, serves several important biosynthetic pathways, including lipogenesis and cholesterogenesis. In nervous tissue, ATP citrate-lyase may be involved in the biosynthesis of acetylcholine. NDPK has been found to phosphorylate ACLY and insulin to increase phosphorylation of ACLY (2).
Related Pathway

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WB Image
Western blot - ATP citrate lyase antibody [EP737Y]; All lanes : Anti-ATP citrate lyase (phospho T447 + S451) antibody [EP737Y] at 1/1000 dilution.Lane 1 : (A) 3T3 cell lysate (untreated cells).Lane 2 : (B) 3T3 cell lysate (insulin treated cells).Lysates/proteins at 10 µg per lane.Secondary.goat anti-rabbit HRP labelled at 1/2000 dilution.Predicted band size : 122 kDa.Observed band size : 100 kDa .
IF Image
Immunocytochemistry/ Immunofluorescence - ATP citrate lyase (phospho T447 + S451) antibody [EP737Y]; TA300817 stained HeLa cells at 1/100-1/250 dilution.

 

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