Invalid object name 'antibody_LocusMapping'. Anti-ACLY Antibody, EP704Y - OriGene
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Anti-ACLY Antibody EP704Y

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA300619
  • Rabbit Monoclonal Antibody against ACLY (Clone EP704Y)
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC420122) , 20ug Explanation
100ul 325 In Stock
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WB(1)
IP(1)
IF(1)
FC(1)
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Also for ACLY (NM_001096)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenA synthetic peptide corresponding to residues in the C-term of human ATP citrate lyase was used as immunogen.
Clone NameEP704Y IsotypeIgG
Species ReactivityMouse, Rat, Human, Marmoset (common) ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, IF, IP, FC Suggested DilutionsICC/IF: Use a concentration of 5 ug/ml; IHC-P: 1:100; WB: 1:1000 - 1:5000; FC: 1:100; IP: Use at an assay dependent concentration
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification IgG fraction

Reference Data

Target NameHomo sapiens ATP citrate lyase (ACLY), transcript variant 1
Alternative NameACL; ATPCL; CLATP
Database LinkNP_001087
FunctionATP citrate lyase (ACL) is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA in many tissues. The enzyme is a tetramer of four identical subunits. It catalyzes the formation of acetyl-CoA and oxaloacetate from citrate and CoA with a concomitant hydrolysis of ATP to ADP and phosphate. One of these products, acetyl-CoA, serves several important biosynthetic pathways, including lipogenesis and cholesterogenesis. In nervous tissue, ATP citrate-lyase may be involved in the biosynthesis of acetylcholine. NDPK has been found to phosphorylate ACL and insulin to increase phosphorylation of ACL (2).
Related Pathway

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot - ATP citrate lyase antibody [EP704Y]; Anti-ATP citrate lyase antibody [EP704Y] at 1/5000 dilution + Hela cell lysate.Predicted band size : 122 kDa.Observed band size : 122 kDa.
IF Image
Immunocytochemistry/ Immunofluorescence - Anti-ATP citrate lyase antibody [EP704Y]; ICC/IF image of TA300619 stained MCF7 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody overnight at +4°C. The secondary antibody (green) was Alexa Fluor 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43uM.
IP Image
Immunoprecipitation - Anti-ATP citrate lyase antibody [EP704Y]; AMPK gamma 1 was immunoprecipitated using 0.5mg Jurkat whole cell extract, 10ug of Rabbit monoclonal [Y308] to AMPK gamma 1 and 50ul of protein G magnetic beads (+). No antibody was added to the control (-). .The antibody was incubated under agitation with Protein G beads for 10min, Jurkat whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.Proteins were eluted by addition of 40ul SDS loading buffer and incubated for 10min at 70oC; 10ul of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with .Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) .Band: 122kDa: ATP citrate lyase; non specific - 60kDa: We are unsure as to the identity of this extra band.
FC Image
Flow Cytometry - ATP citrate lyase antibody [EP704Y]; Overlay histogram showing HeLa cells stained with TA300619 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was DyLight 488 goat anti-rabbit IgG (H+L) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1ug/1x10^6 cells) used under the same conditions. Acquisition of >5,000 events was performed.

 

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