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Home Antibody All anti-ACLY antibodies

Anti-ACLY Antibody EP704Y

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA300619
  • Rabbit Monoclonal Antibody against ACLY (Clone EP704Y)
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC420122) , 20ug Explanation
100ul 325 In Stock
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WB(1)
IP(1)
IF(1)
FC(1)
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Also for ACLY (NM_001096)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenA synthetic peptide corresponding to residues in the C-term of human ATP citrate lyase was used as immunogen.
Clone NameEP704Y IsotypeIgG
Species ReactivityMouse, Rat, Human, Marmoset (common) ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, IF, IP, FC Suggested DilutionsICC/IF: Use a concentration of 5 ug/ml; IHC-P: 1:100; WB: 1:1000 - 1:5000; FC: 1:100; IP: Use at an assay dependent concentration
Predicted MW Explanation 122 kDa
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification IgG fraction

Reference Data

Target NameHomo sapiens ATP citrate lyase (ACLY), transcript variant 1
Alternative NameACL; ATPCL; CLATP
Database LinkNP_001087
Entrez Gene 47 Human
Entrez Gene 104112 Mouse
Entrez Gene 24159 Rat
FunctionATP citrate lyase (ACL) is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA in many tissues. The enzyme is a tetramer of four identical subunits. It catalyzes the formation of acetyl-CoA and oxaloacetate from citrate and CoA with a concomitant hydrolysis of ATP to ADP and phosphate. One of these products, acetyl-CoA, serves several important biosynthetic pathways, including lipogenesis and cholesterogenesis. In nervous tissue, ATP citrate-lyase may be involved in the biosynthesis of acetylcholine. NDPK has been found to phosphorylate ACL and insulin to increase phosphorylation of ACL (2).
Related PathwayDruggable Genome Citrate cycle (TCA cycle)Metabolic pathways

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot - ATP citrate lyase antibody [EP704Y]; Anti-ATP citrate lyase antibody [EP704Y] at 1/5000 dilution + Hela cell lysate.Predicted band size : 122 kDa.Observed band size : 122 kDa.
IF Image
Immunocytochemistry/ Immunofluorescence - Anti-ATP citrate lyase antibody [EP704Y]; ICC/IF image of TA300619 stained MCF7 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody overnight at +4°C. The secondary antibody (green) was Alexa Fluor 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43uM.
IP Image
Immunoprecipitation - Anti-ATP citrate lyase antibody [EP704Y]; AMPK gamma 1 was immunoprecipitated using 0.5mg Jurkat whole cell extract, 10ug of Rabbit monoclonal [Y308] to AMPK gamma 1 and 50ul of protein G magnetic beads (+). No antibody was added to the control (-). .The antibody was incubated under agitation with Protein G beads for 10min, Jurkat whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.Proteins were eluted by addition of 40ul SDS loading buffer and incubated for 10min at 70oC; 10ul of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with .Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) .Band: 122kDa: ATP citrate lyase; non specific - 60kDa: We are unsure as to the identity of this extra band.
FC Image
Flow Cytometry - ATP citrate lyase antibody [EP704Y]; Overlay histogram showing HeLa cells stained with TA300619 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was DyLight 488 goat anti-rabbit IgG (H+L) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1ug/1x10^6 cells) used under the same conditions. Acquisition of >5,000 events was performed.

 

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