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Anti-ACLY Antibody EP704Y
Also for ACLY (NM_001096)
|A synthetic peptide corresponding to residues in the C-term of human ATP citrate lyase was used as immunogen.|
|Mouse, Rat, Human, Marmoset (common)
||Lot dependent; please refer to CoA along with shipment
|WB, IF, IP, FC
||ICC/IF: Use a concentration of 5 ug/ml; IHC-P: 1:100; WB: 1:1000 - 1:5000; FC: 1:100; IP: Use at an assay dependent concentration
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Homo sapiens ATP citrate lyase (ACLY), transcript variant 1|
|ACL; ATPCL; CLATP|
Entrez Gene 47 Human
Entrez Gene 104112 Mouse
Entrez Gene 24159 Rat
|ATP citrate lyase (ACL) is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA in many tissues. The enzyme is a tetramer of four identical subunits. It catalyzes the formation of acetyl-CoA and oxaloacetate from citrate and CoA with a concomitant hydrolysis of ATP to ADP and phosphate. One of these products, acetyl-CoA, serves several important biosynthetic pathways, including lipogenesis and cholesterogenesis. In nervous tissue, ATP citrate-lyase may be involved in the biosynthesis of acetylcholine. NDPK has been found to phosphorylate ACL and insulin to increase phosphorylation of ACL (2).|
|Druggable Genome Citrate cycle (TCA cycle)Metabolic pathways|
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Western blot - ATP citrate lyase antibody [EP704Y]; Anti-ATP citrate lyase antibody [EP704Y] at 1/5000 dilution + Hela cell lysate.Predicted band size : 122 kDa.Observed band size : 122 kDa.
ICC/IF image of TA300619 stained MCF7 cells. The cells were incubated with the antibody overnight at 4.. The secondary antibody (green) was Alexa Fluor 488 goat anti-rabbit IgG (H+L) used at 1:1000 for 1h. Alexa Fluor 594 WGA was used to label plasma membranes (red) at 1:200 for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43uM.
AMPK gamma 1 was immunoprecipitated using 0.5mg Jurkat whole cell extract, 10ug of Rabbit monoclonalto AMPK gamma 1 and 50ul of protein G magnetic beads (+). No antibody was added to the control (-). Secondary: Mouse monoclonalSecondary Antibody to Rabbit IgG light chain (HRP) .Band: 122kDa: ATP citrate lyase; non specific - 60kDa: We are unsure as to the identity of this extra band.
Flow Cytometry - ATP citrate lyase antibody; Overlay histogram showing HeLa cells stained with TA300619 (red line). The secondary antibody used was DyLight 488 goat anti-rabbit IgG (H+L) at 1:500. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1ug/1x10^6 cells) used under the same conditions. Acquisition of >5,000 events was performed.