Comparison of the dose–response relationship of radiation-induced apoptosis in the hippocampal dentate gyrus and intestinal crypt of adult mice
Radiat Prot Dosimetry, Mar 2012; 148: 492 - 497.
[LGR5
]
Tracking protein aggregation and mislocalization in cells with flow cytometry
Nature Methods doi:10.1038/nmeth.1930
[HSPA1A]
Histone deacetylase inhibitors prevent the degradation and restore the activity of glucocerebrosidase in Gaucher disease
PNAS, Dec 2011; 108: 21200 - 21205.
[anti-DDK antibody]
A synthetic peptide corresponding to residues surrounding serine 221 of human ACC2 was used as an immunogen.
Buffer
Store at -20C. Buffer: 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. Stable for 12 months from date of receipt.
Acetyl-CoA carboxylase 2 (ACC2) is a biotin-dependent enzyme that is involved in the regulation of fatty acid oxidation, rather than fatty acid biosynthesis. ACC2 is predominantly expressed in the heart, skeletal muscles and liver (1). It catalyzes the irreversible carboxylation of acetyl-CoA to produce malonyl-CoA through its two catalytic activities, biotin carboxylase and carboxyltransferase. ACC2 is throught to control fatty acid oxidation by the means of the ability of malonyl-CoA to inhibit carnitine-palmitoyl-CoA transferase I (CPT-1), the rate-limiting step in fatty acid uptake and oxidation by mitochondria (2). The activity of ACC2 is controlled by reversible phosphorylation. Ser219 and Ser221 were found to be critical for the phosphorylation and subsequent inactivation of ACC2 (3).
Related Pathway
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