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Anti-ACACA Antibody EP687Y
Also for ACACA (NM_198834)
|A synthetic peptide corresponding to residues in the human Acetyl-CoA carboxylase 1 was used as an immunogen.|
|Human, Mouse, Rat
||Lot dependent; please refer to CoA along with shipment
||WB: 1:2000; IHC-P: Use at an assay dependent dilution
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant
|Is unsuitable for Flow Cyt,ICC/IF or IP.
|Homo sapiens acetyl-CoA carboxylase alpha (ACACA), transcript variant 1|
|ACAC; ACACAD; ACC; ACC1; ACCA|
|Acetyl-CoA carboxylase (ACC) is a complex multifunctional enzyme system. ACC is a biotin-containing enzyme which catalyzes the carboxylation of acetyl-CoA to malonyl-CoA, the rate-limiting step in fatty acid synthesis. There are two ACC forms, alpha and beta, encoded by two different genes. ACC-alpha is highly enriched in lipogenic tissues. The enzyme is under long term control at the transcriptional and translational levels and under short term regulation by the phosphorylation/dephosphorylation of targeted serine residues and by allosteric transformation by citrate or palmitoyl-CoA. Multiple alternatively spliced transcript variants divergent in the 5' sequence and encoding distinct isoforms have been found for this gene. [provided by RefSeq]. |
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Western blot - Acetyl Coenzyme A Carboxylase antibody [EP687Y]; Anti-Acetyl Coenzyme A Carboxylase antibody [EP687Y] at 1/2000 dilution + A431 cell lysate at 10 µg.Secondary.Goat anti-rabbit HRP labelled, at 1/2000 dilution.Predicted band size : 265 kDa.Observed band size : 265 kDa.
Immunohistochemistry (Paraffin-embedded sections) - Acetyl Coenzyme A Carboxylase antibody [EP687Y]; Ab45174, at a dilution of 1/50, staining human Acetyl Coenzyme A Carboxylase in human liver by immunohistochemistry using paraffin embedded tissue.