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Home Antibody All anti-ABAT antibodies

Anti-ABAT Antibody EPR4433

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SKU Description Amount Price Availability*  
TA307681 Rabbit monoclonal antibody against ABAT(clone EPR4433) 100ul $325 In Stock
LC400218 ABAT HEK293T cell transient overexpression lysate (as WB positive control) 20ug $50 In Stock

Buy any antibody of 100ul or more, get a free package of 3 loading control antibody samples. View Details Add to Shopping Cart

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WB(1)
IHC(1)
FC(1)

OriGene Data

ImmunogenA synthetic peptide corresponding to residues in human ABAT was used as an immunogen.
Clone NameEPR4433 IsotypeIgG
Species ReactivityMouse, Rat, Human ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, IHC, FC Suggested DilutionsWB: 1:10000 - 1:50000; IP: 1:10 - 1:100; IHC-P: 1:250 - 1:500; FC: 1:100
Predicted MW Explanation 56 kDa
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification Tissue culture supernatant

Reference Data

Target NameHomo sapiens 4-aminobutyrate aminotransferase (ABAT), transcript variant 2
Alternative NameGABA-AT; GABAT; NPD009
Database LinkNP_000654
Entrez Gene 18 Human
Entrez Gene 268860 Mouse
Entrez Gene 81632 Rat
FunctionABAT (4-aminobutyrate aminotransferase) is responsible for catabolism of gamma-aminobutyric acid (GABA), an important, mostly inhibitory neurotransmitter in the central nervous system, into succinic semialdehyde. The active enzyme is a homodimer of 50-kD subunits complexed to pyridoxal-5-phosphate. GABA is estimated to be present in nearly one-third of human synapses. ABAT in liver and brain is controlled by 2 codominant alleles with a frequency in a Caucasian population of 0.56 and 0.44. The ABAT deficiency phenotype includes psychomotor retardation, hypotonia, hyperreflexia, lethargy, refractory seizures, and EEG abnormalities (1).
Related PathwayDruggable Genome Alanine, aspartate and glutamate metabolismValine, leucine and isoleucine degradationbeta-Alanine metabolismPropanoate metabolismButanoate metabolismMetabolic pathwaysMore Pathways >>

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

Western blot - ABAT antibody [EPR4433]; All lanes : Anti-ABAT antibody [EPR4433].Lane 1 : HepG2 cell lysate.Lane 2 : SH-SY5Y cell lysate.Lane 3 : Human fetal liver tissue lysate.Lane 4 : rat brain tissue lysate.Lane 5 : rat kidney tissue lysate.Lane 6 : mouse brain tissue lysate.Predicted band size : 56 kDa.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - ABAT antibody [EPR4433]; TA307681, at 1/250 dilution, staining ABAT in Human liver tissue by immunohistochemistry.
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Flow Cytometry - Anti-ABAT antibody [EPR4433]; Overlay histogram showing HepG2 cells stained with TA307681 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody for 30 min at 22°C. The secondary antibody used was DyLight 488 goat anti-rabbit IgG (H+L) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1ug/1x10^6 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HepG2 cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
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