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OriGene 4C5 anti-DDK in recent publications
Superoxide dismutase 1 (SOD1) is a target for a small molecule identified in a screen for inhibitors of the growth of lung adenocarcinoma cell lines PNAS, Sep 2011; 108: 16375 - 16380. [DDK]

Ectopic expression of cadherin 8 is sufficient to cause cyst formation in a novel 3D collagen matrix renal tubule culture Am J Physiol Cell Physiol, Jul 2011; 301: C99 - C105. [DDK]

Purification, Identification, and Cloning of Lysoplasmalogenase, the Enzyme That Catalyzes Hydrolysis of the Vinyl Ether Bond of Lysoplasmalogen J. Biol. Chem., Jul 2011; 286: 24916 - 24930. [DDK]

Heparan Sulfate Modification of the Transmembrane Receptor CD47 Is Necessary for Inhibition of T Cell Receptor Signaling by Thrombospondin-1 J. Biol. Chem., Apr 2011; 286: 14991 - 15002 [DDK]

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4C5 Anti-DDK Monoclonal Antibody

AntiDDK Western Blot Image

OriGene has developed 4C5 anti-DDK antibody for the detection of DYKDDDDK epitope (same epitope as Flag®)

  • Validated for Western blot, immunoprecipitation, immunofluorescence and flow cytometry
  • Higher sensitivity and specificity compared to other vendors’ anti-flag antibodies
  • Purify recombinant proteins with high purities and yields
  • Detect recombinant proteins with low expression levels

4C5 anti-DDK antibody has already been used to

  • Validate the recombinant protein expression from more than 15,000 OriGene TrueORF cDNA clones and VERIFY overexpression lysates
  • Purify more than 5,000 human recombinant proteins from HEK293 cells
  • Purify immunogens under native conditions for the development of TrueMAB antibodies
SKU Description Data Sheet Price Shipping  
TA50011-100 4C5, Anti-DDK monoclonal antibody (100 µl) pdf $248 Immediate Add to Shopping Cart
TA50011-1 4C5, Anti-DDK monoclonal antibody (1 ml) pdf $590 Immediate Add to Shopping Cart
TA50011-5 4C5, Anti-DDK monoclonal antibody (5 ml) pdf $1700 Immediate Add to Shopping Cart
TA150015 4C5, Anti-DDK monoclonal antibody, Biotin (200 ul) pdf $318 Immediate Add to Shopping Cart
TA150030 4C5, Anti-DDK Monoclonal antibody, HRP (100 μg) pdf $318 Immediate Add to Shopping Cart
TA150020 4C5, Anti-DDK monoclonal antibody, Phycoerythrin (PE) pdf $318 Immediate Add to Shopping Cart
TA150021 4C5, Anti-DDK monoclonal antibody, DyLight 488 pdf $318 Immediate Add to Shopping Cart
TA150022 4C5, Anti-DDK monoclonal antibody, DyLight 550 pdf $318 Immediate Add to Shopping Cart
 
TA150014 Anti-Myc and Anti-DDK monoclonal antibodies (2 tubes @ 50 μL each) pdf $248 Immediate Add to Shopping Cart

* For bulk antibody package, please contact Tech Support
** Peptide sequence of the DDK-tag (Flag®): N-DYKDDDDK-C
*** Flag® is a trademark of Sigma-Aldrich.

AntiDDK IF Image AntiDDK FC Image AntiDDK DDK Comparison Image
Figure 4. 4C5 anti-DDK monoclonal antibody (TA50011) showed higher sensitivity in the side-by-side comparison with M2 monoclonal antibody from vendor S. Both antibodies were used at 1:2000 dilution against same sample (HSPA9) dilutions.

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