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Human Equilibrative Nucleoside Transporter ENT1 Stable Cell Line - HeLa
SLC29A1 Other names: ENT1
Cryopreserved cells, 3 x 10E6 cells/vial
Negative by MycoAlert Kit, Lonza
Cell Line Validation:
Equilibrative Nucleoside Transporter (ENT1), is a member of the equilibrative nucleoside transporter family. The gene encodes a transmembrane glycoprotein that localizes to the plasma and mitochondrial membranes and mediates the cellular uptake of nucleosides from the surrounding medium. The protein is categorized as an equilibrative (as opposed to concentrative) transporter that is sensitive to inhibition by nitrobenzylthioinosine (NBMPR). Nucleoside transporters are required for nucleotide synthesis in cells that lack de novo nucleoside synthesis pathways, and are also necessary for the uptake of cytotoxic nucleosides that are used for cancer and viral chemotherapies.
Figure 1. Immunoprecipation analysis in HeLa/ENT1 by using mAb-agrose against DDK (FLAG) tag (Cat# TA50011-100). 15 ug of membrane protein was used for this immunoprecipation. Membrane protein concentrations were measured by BCA kit (Thermo Scientific Inc.). Lane A: Membrane protein from HeLa/ENT1-pCMV Entry stable cell line. The specific band between 64KD and 97 KD is ENT1. The 25KD band is the mAb light chain and the 50KD band is the heavy chain. Lane B: Membrane protein from NIH3T3 cells tranfected with pCMV-Entry vectors was used as negative control. Lane C: Membrane protein from NIH3T3 cells was used as negative control.