OriGene Technologies, Inc.
Search:    
Left ProductsProducts divider ServicesServices divider technologyTechnology divider researchResearch divider TechsupportTechSupport divider AboutAbout Right

Video overview of cDNA clone offering
Video brochure for cDNA clone
Related Products

Over 1000 citations of OriGene cDNA clones
Nuc-ErbB3 regulates H3K27me3 levels and HMT activity to establish epigenetic repression during peripheral myelination Glia Jun 2016 [Pou3f2]

PKCe inhibits isolation and stemness of side population cells via the suppression of ABCB1 transporter and PI3K/Akt, MAPK/ERK signaling in renal cell carcinoma cell line 769P Cancer Lett. Jun 2016 [PRKCE]

AIG1 and ADTRP are atypical integral membrane hydrolases that degrade bioactive FAHFAs Nat. Chem. Biol. May 2016 [ADTRP]

miR-613 inhibits the growth and invasiveness of human hepatocellular carcinoma via targeting DCLK1 Biochem. Biophys. Res. Commun. May 2016 [DCLK1]

View All Citations >>

 

SLC29A1 (NM_001078177 ) Stable Cell Line

Catalog #:SL500074Price:$12888    technical questions?
Description:Human Equilibrative Nucleoside Transporter ENT1 Stable Cell Line - HeLa
Gene:SLC29A1
Other names: ENT1
Host CellHeLa
cDNA Clone:
Format:Cryopreserved cells, 3 x 10E6 cells/vial
Mycoplasma:Negative by MycoAlert Kit, Lonza
Cell Line Validation:Western blot
Background:Equilibrative Nucleoside Transporter (ENT1), is a member of the equilibrative nucleoside transporter family. The gene encodes a transmembrane glycoprotein that localizes to the plasma and mitochondrial membranes and mediates the cellular uptake of nucleosides from the surrounding medium. The protein is categorized as an equilibrative (as opposed to concentrative) transporter that is sensitive to inhibition by nitrobenzylthioinosine (NBMPR). Nucleoside transporters are required for nucleotide synthesis in cells that lack de novo nucleoside synthesis pathways, and are also necessary for the uptake of cytotoxic nucleosides that are used for cancer and viral chemotherapies.
Figure 1. Immunoprecipation analysis in HeLa/ENT1 by using mAb-agrose against DDK (FLAG) tag (Cat# TA50011-100). 15 ug of membrane protein was used for this immunoprecipation. Membrane protein concentrations were measured by BCA kit (Thermo Scientific Inc.). Lane A: Membrane protein from HeLa/ENT1-pCMV Entry stable cell line. The specific band between 64KD and 97 KD is ENT1. The 25KD band is the mAb light chain and the 50KD band is the heavy chain. Lane B: Membrane protein from NIH3T3 cells tranfected with pCMV-Entry vectors was used as negative control. Lane C: Membrane protein from NIH3T3 cells was used as negative control.

Related Products

 

patter
Inc 5000 Healthcare Company
All Products by: Title | Price | Category | Popularity | Best Sellers Topselling Products by: Title | Price | Category | Popularity | Favorites
Popular Categories: Popularity | Our Choices | All-Round Favorites | Title Topselling Categories: Popularity | Our Choices | All-Round Favorites | Title