15-PGDH inhibits hepatocellular carcinoma growth through 15-keto-PGE2/PPAR?-mediated activation of p21WAF1/Cip1 Oncogene doi:10.1038/onc.2013.69
[PTGR2 ]
A Modified Form of Diphthamide Causes Immunotoxin Resistance in a Lymphoma Cell Line with a Deletion of the WDR85 Gene J. Biol. Chem., Apr 2013; 288: 12305 - 12312.
[WDR85]
A New Signaling Pathway (JAK-Fes-phospholipase D) That Is Enhanced in Highly Proliferative Breast Cancer Cells J. Biol. Chem., Apr 2013; 288: 9881 - 9891.
[FES]
Androgen receptor inclusions acquire GRP78/BiP to ameliorate androgen-induced protein misfolding stress in embryonic stem cells Cell Death and Disease 4, e607 doi:10.1038/cddis.2013.122
[GRP78]
Human Doublecortin-like Kinase 1 DCAMKL1 Stable Cell Line - NIH3T3
Gene:
DCLK1 Other names: CL1; CLICK1; DCAMKL1; DCDC3A; DCLK
Host Cell
NIH3T3
cDNA Clone:
Format:
Cryopreserved cells, 2 x 10E6 cells/vial
Mycoplasma:
Negative by MycoAlert Kit, Lonza
Cell Line Validation:
Western blot
Background:
Doublecortin-like Kinase 1 (DCAMKL1) is a member of the protein kinase superfamily and the doublecortin family. It contains two N-terminal doublecortin domains, a C-terminal serine/threonine protein kinase domain and a serine/proline-rich domain which is between the doublecortin and the protein kinase domains. Different domains reveal alterative functions. N-terminal domains bind microtubules and regulate microtubule polymerization. The C-terminal domain shows substantial homology to Ca2+/calmodulin-dependent protein kinase. However, the serine/proline-rich domain functions in mediating multiple protein-protein interactions. DCAMKL1 involves in several different cellular processes, including neuronal migration, retrograde transport, neuronal apoptosis and neurogenesis. DCAMKL1 expression is up-regulated by brain-derived neurotrophic factor and is associated with memory and general cognitive abilities.
Figure 1. Western blot analysis for NIH3T3/ DCAMKL1 using mAb against DDK (FLAG) tag (Cat# TA50011-100). Protein concentrations were measured by BCA kit (Thermo Scientific Inc.). Lane A: NIH3T3/ DCAMKL1 Lysate at Passage 1 from Master Cell Bank, 15 ug; Lane B: NIH3T3/ DCAMKL1 Lysate at Passage 6 from Master Cell Bank, 15 ug ; Lane C: Untransfected NIH3T3 cells Lysate was used as negative control, 15 ug.
