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Goat Anti-Rabbit Secondary Antibody
|Product Name||Peroxidase Conjugated Goat Anti-Rabbit IgG (Min Hu, Ms)|
|Immunogen||Rabbit IgG, whole molecule|
|Application||WB: 1:3,000-10,000; ELISA: 1:20,000-40,000|
|Buffer||0.01M PBS (pH7.4)|
|Purification||This antibody was purified from antiserum by immunoaffinity chromatography which removes essentially all goat serum proteins, except the specific antibody for rabbit IgG. |
|Storage||Store lyophilized powders at 2-8C. When ready to use, reconstitute with 0.5ml dH2O and centrifuge briefly. Product is stable at 2-8C for 6 weeks as undiluted liquid. Prepare small aliquots to avoid repeated freezing and thawing. For extended storage after rehydration, aliquot and store at -20C or below. Product will be state for one year after rehydration. Alternatively, add an equal volume of glycerol (ACS grade or better) for a final concentration of 50% and store at -20C. Please note that, adding glycerol reduces the stated protein concentration by half and the dilution ratio should be adjusted accordingly.|
|Note||This peroxidase conjugated antibody is specific for rabbit IgG and shows no cross-reactivity other animal species IgG proteins.|
West blot analysis of GAPDH expression in 9 cell lines using rabbit anti-GAPDH as primary antibody and HRP conjugated goat anti-rabbit IgG as secondary antibody (TA140003).
ELISA analysis of rabbit IgG with HRP-conjugated goat anti-rabbit secondary (TA140003) or with a comparable product from Company J.
ELISA analysis of DDK (FLAG tag) with different dilutions of rabbit anti-DDK primary antibody and anti-rabbit secondary antibody. 1) Dark Green: OriGene (OTI) HRP-conjugated goat anti-rabbit secondary (TA140003 diluted at 1:20,000); 2) Light Green: TA140003 diluted at 1:40,000; 3) Red: a comparable secondary antibody from Company J.