Abcb1a (NM_133401) Rat Untagged Clone

CAT#: RN211714

Abcb1a (untagged ORF) - Rat ATP-binding cassette, sub-family B (MDR/TAP), member 1 (Abcb1), (10 ug)

Specifications

Product Data

• Type: Rat Untagged Clone
• Tag: Tag Free
• Symbol: Abcb1a
• Synonyms: Abcb1; Mdr1a
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >RN211714 representing NM_133401
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGCTCGAAGAAGACCTTAACGGAAGAGCAGACAAGAACTTCTCAAAGATGGGCAAAAAGAGTAAAA
  AGGAGAAGAAAGAAAAGAAACCAGCGGTCAGTGTGCTCACAATGTTTCGCTATGCAGGTTGGCTGGACAG
  ATTTTACATGCTGCTGGGAACTCTGGCGGCCATTATCCATGGAATTGCGCTCCCACTTATGATGCTGGTC
  TTTGGAGACATGACAGATAGCTTTGCAAATGTAGGAAACAACCGTAGTATGAGTTTCTACAATGCTACAG
  ACATATATGCCAAGCTGGAGGACGAAATGACCACGTACGCCTACTATTACACGGGCATTGGTGCCGGTGT
  GCTCATCGTTGCCTACATCCAGGTTTCACTTTGGTGCCTGGCAGCTGGGAGACAAATACACAAGATTAGG
  CAGAAGTTTTTCCATGCCATCATGAATCAGGAGATAGGCTGGTTTGACGTGCATGACGTTGGGGAGCTCA
  ACACCCGGCTCACAGATGACGTCTCCAAAATTAATGAAGGAATTGGTGACAAAATTGGAATGTTCTTTCA
  GGCAATGGCAACATTTTTTGGTGGTTTTATAATAGGATTTACTCGCGGCTGGAAGCTAACTCTTGTGATT
  TTGGCCATCAGCCCTGTTCTTGGACTGTCAGCTGGTATTTGGGCAAAGATATTGTCTTCATTTACTGATA
  AGGAACTCCAGGCTTATGCAAAAGCTGGAGCAGTTGCTGAAGAAGTCTTAGCAGCCATCAGAACTGTGAT
  TGCCTTTGGAGGACAAAAGAAGGAACTTGAAAGGTACAATAACAATTTGGAAGAAGCTAAAAGGCTTGGG
  ATAAAGAAAGCTATCACGGCCAACATTTCCATGGGTGCAGCTTTTCTGCTTATCTATGCATCATATGCTC
  TGGCATTCTGGTATGGGACTTCCTTGGTCATCTCAAAAGAATACACTATTGGACAAGTGCTCACTGTCTT
  TTTTTCTGTATTAATTGGAGCATTCAGTGTTGGGCAGGCATCTCCAAATATTGAAGCCTTCGCCAATGCT
  AGAGGAGCAGCTTATGAAGTCTTCAGTATAATTGATAATAAGCCCAGTATAGACAGCTTCTCAAAGAGTG
  GGCACAAACCCGACAACATACAAGGAAATTTGGAATTCAAAAATATTCACTTCAGTTACCCGTCTCGAAA
  AGACGTTCAGATCTTGAAGGGCCTCAACCTGAAGGTGAAGAGCGGGCAGACGGTAGCCCTGGTTGGCAAC
  AGTGGCTGTGGGAAAAGCACAACTGTCCAGCTGCTGCAGAGGCTCTACGACCCCATAGAGGGCGAGGTCA
  GTATCGACGGACAGGACATCAGGACCATCAATGTGAGGTATCTGCGGGAAATCATTGGGGTGGTGAGTCA
  GGAACCCGTGCTGTTTGCCACCACAATTGCCGAAAACATTCGCTATGGCCGAGAAAACGTCACCATGGAT
  GAGATAGAGAAAGCTGTCAAGGAAGCCAATGCCTATGATTTCATCATGAAACTGCCCCACAAATTTGACA
  CCCTGGTTGGTGAGAGAGGGGCGCAGCTGAGTGGGGGACAGAAACAGAGGATCGCCATTGCCCGGGCCCT
  GGTCCGCAACCCCAAGATCCTTTTGTTGGATGAGGCCACGTCAGCCTTGGACACAGAAAGCGAAGCCGTG
  GTTCAGGCCGCTCTGGATAAGGCTAGAGAAGGCCGGACCACCATTGTGATAGCTCACCGCTTGTCTACAG
  TTCGCAATGCTGACGTCATTGCTGGTTTTGATGGTGGTGTCATTGTGGAGCAAGGAAATCATGATGAGCT
  CATGAGAGAGAAAGGAATTTACTTCAAACTTGTCATGACTCAGACAGCAGGAAATGAAATTGAATTAGGA
  AATGAAGCTTGTGAATCTAAAGATGGAATTGATAATGTGGACATGTCTTCAAAAGATTCAGGATCCAGTC
  TAATAAGAAGAAGATCAACTCGCAAAAGCATCCGTGGGCCACATGATCAAGACGGGGAACTTAGCACCAA
  AGAGGCTCTGGATGACGACGTACCTCCAGCTTCCTTTTGGCGGATCCTGAAGTTGAATTCAACTGAATGG
  CCTTATTTTGTGGTTGGTGTATTTTGTGCCATAATAAATGGAGGCTTGCAACCAGCATTCTCCATAATAT
  TTTCAAAGGTTGTAGGGGTTTTTACAAAAAATGACACCCCTGAAATCCAGCGGCAGAACAGCAACTTGTT
  TTCTTTATTGTTCCTGATCCTTGGGATCATCTCTTTCATTACGTTTTTCCTTCAGGGCTTCACATTTGGC
  AAAGCTGGAGAGATCCTCACCAAGCGACTCCGATACATGGTCTTCAAATCCATGCTGAGACAGGACATAA
  GCTGGTTTGATGACCCTAAAAACACCACAGGAGCGCTGACCACCAGGCTTGCCAATGACGCTGCTCAAGT
  GAAAGGGGCTACAGGGTCTAGGCTTGCTGTTATTACCCAGAACATAGCAAATCTTGGGACAGGCATCATC
  ATATCCCTGATCTACGGCTGGCAATTGACACTTTTACTCCTAGCAATTGTTCCCATCATTGCTATAGCAG
  GAGTGGTTGAAATGAAAATGTTGTCTGGACAAGCGCTGAAAGATAAGAAGGAACTAGAAGGTTCTGGGAA
  GATCGCTACAGAAGCAATTGAAAACTTTCGCACTGTCGTCTCTTTGACTCGGGAGCAGAAGTTTGAAACT
  ATGTATGCCCAGAGCTTGCAGATACCATACAGAAATGCTTTGAAGAAAGCGCACGTCTTTGGGATCACTT
  TCTCCTTCACCCAGGCCATGATGTATTTCTCCTATGCTGCTTGTTTCCGGTTTGGTGCCTACTTGGTGGC
  ACGAGAACTCATGACATTTGAAAATGTTCTGTTAGTATTCTCAGCTATTGTCTTTGGTGCCATGGCAGTG
  GGGCAGGTCAGTTCATTCGCTCCTGACTACGCGAAAGCCAAAGTCTCGGCATCCCACATCATCAGGATCA
  TTGAGAAAATCCCTGAGATTGACAGCTACAGCACGGAGGGCTTGAAGCCTAATATGTTGGAAGGAAATGT
  GAAATTTAATGGAGTCATGTTCAACTATCCCACCCGACCCAACATCCCAGTGCTTCAGGGGCTGAGCCTA
  GAGGTGAAGAAAGGGCAGACGCTGGCCCTCGTGGGCAGCAGTGGCTGCGGGAAGAGTACAGTGGTCCAGC
  TGCTTGAGCGCTTCTATGACCCCATGGCCGGAACAGTGTTTCTAGATGGCAAAGAAATAAAGCAACTCAA
  TGTCCAGTGGCTCCGCGCCCACCTGGGCATTGTGTCCCAGGAGCCCATCCTGTTTGACTGCAGCATCGCC
  GAGAACATTGCCTACGGAGACAACAGCCGTGTCGTGTCTCATGAGGAGATCGTGAAGGCAGCCAAGGAGG
  CCAACATCCACCAGTTCATCGACTCACTGCCTGAGAAATACAACACCAGAGTGGGAGACAAAGGGACTCA
  GCTGTCGGGCGGGCAGAAGCAGCGCATCGCCATCGCGCGCGCCCTCGTCAGACAGCCTCACATCTTACTT
  CTGGATGAAGCGACATCAGCTCTGGATACGGAGAGTGAAAAGGTCGTCCAGGAAGCGCTGGACAAAGCCA
  GGGAAGGCCGCACCTGCATTGTGATCGCGCACCGCCTGTCCACCATCCAGAACGCAGACTTGATCGTGGT
  GATTCAGAACGGCCAGGTCAAGGAGCACGGCACCCACCAGCAGCTGCTGGCCCAGAAAGGCATCTATTTC
  TCGATGGTCAGTGTGCAGGCTGGAGCAAAGCGCTCATGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_133401
• Insert Size: 3819 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_133401.1, NP_596892.1
• RefSeq Size: 4927 bp
• RefSeq ORF: 3819 bp
• Locus ID: 170913
• Cytogenetics: 4q12
• Gene Summary: ATP-binding cassette (ABC) multiple drug transporter P-glycoprotein that is activated during liver regeneration and hepatocarcinogenesis [RGD, Feb 2006]