Abcg1 (NM_053502) Rat Untagged Clone

CAT#: RN202746

Abcg1 (untagged ORF) - Rat ATP-binding cassette, sub-family G (WHITE), member 1 (Abcg1), (10 ug)

Specifications

Product Data

• Type: Rat Untagged Clone
• Tag: Tag Free
• Symbol: Abcg1
• Synonyms: Abc8
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >RN202746 representing NM_053502
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCTGTCTGATGGCCGCTTTCTCGGTCGGCACCGCCATGAATGCCAGCAACTACTCGGCCGCAATGA
  CGGAACCCAAGTCCGTGTGCGTGTCGGTGGACGAGGTCGTGTCCAGCAACGTGGATGAGCTTGAGACAGA
  TCTGCTTAATGGGCACCTGAAGAAGGTGGACAACAACTTCACGGAGGCCCAGCGCTTTTCCTCCCTGCCG
  CGGAGGGCGGCCGTGAATATCGAATTCAAGGACCTTTCTTACTCCGTACCCGAGGGACCCTGGTGGAGGA
  AGAAAGGATACAAGACCCTTTTGAAAGGGATCTCTGGGAAGTTCAACAGTGGAGAGCTGGTGGCCATCAT
  GGGTCCTTCTGGAGCTGGGAAGTCCACACTCATGAATATTCTGGCGGGATACAGGGAGACGGGCATGAAG
  GGGGCAGTCCTTATCAATGGTATGCCCCGCGACCTGCGCTGCTTCCGGAAGGTCTCGTGCTACATCATGC
  AGGATGACATGCTGCTGCCTCACCTCACTGTCCAGGAGGCCATGATGGTGTCCGCGCATCTGAAGCTGCA
  AGAGAGGGATGAAGGCAGACGGGAGATGGTCAAGGAGATCCTGACAGCACTGGGCTTGCTGCCCTGTGCC
  AACACACGCACAGGGAGCCTCTCGGGTGGCCAGCGGAAGCGCCTGGCCATTGCACTAGAACTGGTCAACA
  ACCCTCCTGTTATGTTCTTTGATGAGCCCACCAGTGGCCTGGACAGTGCCTCTTGCTTCCAAGTGGTGTC
  CCTGATGAAAGGGCTGGCCCAGGGCGGCCGCTCCATCGTCTGCACCATCCACCAACCCAGTGCCAAGCTC
  TTCGAGCTCTTTGACCAGCTTTATGTCCTAAGTCAAGGACAATGTGTCTACCGGGGAAAGGTCTCGAATC
  TCGTGCCGTACCTGAGGGATCTGGGTCTGAACTGCCCTACCTACCACAACCCTGCAGACTTTGTCATGGA
  AGTGGCATCAGGGGAGTACGGTGATCAGAACAGCCGCCTGGTGAGGGCCGTTCGGGAGGGCATGTGTGAC
  TCTGACTATAAGAGAGAACTCGGGGGCGACGGAGACGTGAACCCGTTCCTCTGGCACCGGCCTGCTGAAG
  AGGACTCGGCCTCCATGGAAGGTTGCCACAGCTTCTCGGCCAGCTGCCTCACCCAGTTCTGCATCCTCTT
  CAAGAGGACCTTCCTTAGCATCATGCGGGACTCGGTACTGACACACCTGCGAATCACCTCACACATCGGG
  ATCGGTCTCCTCATTGGCCTCCTGTACCTTGGGATTGGGAACGAAGCCAAGAAGGTCCTGAGCAACTCCG
  GCTTCCTGTTCTTCTCCATGCTGTTCCTCATGTTCGCCGCCCTCATGCCCACCGTTCTGACCTTTCCCCT
  CGAGATGAGTGTCTTCCTCCGAGAGCACCTGAACTACTGGTACAGTCTGAAGGCCTACTACCTGGCCAAG
  ACCATGGCTGATGTCCCCTTTCAGATCATGTTCCCCGTGGCCTACTGCAGCATCGTGTACTGGATGACAT
  CACAGCCGTCGGACGCTGTGCGTTTTGTGCTGTTCGCTGCACTGGGCACCATGACCTCGTTGGTGGCCCA
  GTCCTTAGGCCTACTCATTGGAGCTGCGTCCACATCCCTGCAGGTTGCGACGTTTGTGGGTCCCGTGACA
  GCCATCCCTGTCTTGCTCTTCTCCGGGTTCTTTGTCAGCTTTGACACCATCCCAACCTACCTGCAGTGGA
  TGTCCTACATCTCCTACGTCAGATACGGCTTCGAGGGGGTCATCCTGTCCATCTATGGCTTGGACCGAGA
  GGACCTGCACTGCGACATCGCGGAGACATGCCACTTCCAGAAGTCAGAGGCCATCCTGCGGGAGCTAGAT
  GTGGAGAACGCGAAGCTATACCTGGATTTCATCGTCCTGGGCATCTTCTTCATCTCCCTGCGGCTCATCG
  CCTATTTTGTCCTGAGATACAAAATCCGGGCTGAGAGGTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_053502
• Insert Size: 2001 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_053502.1, NP_445954.1
• RefSeq Size: 3527 bp
• RefSeq ORF: 2001 bp
• Locus ID: 85264
• Cytogenetics: 20p12
• Gene Summary: ATP-binding cassette (ABC) half-transporter that facilitates the efflux of excess cholesterol from macrophages and may play an important role in cholesterol homeostasis [RGD, Feb 2006]